Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Bioresour Technol. 2022 Feb;345:126469. doi: 10.1016/j.biortech.2021.126469. Epub 2021 Dec 2.
This study explored effects of different concentrations of penicillin G on nitrogen conversion, bacterial community composition, and quorum sensing during chicken manure aerobic composting. After composting, adding penicillin G down-regulated the abundance of 71 genera and up-regulated the abundance of 103 genera. These bacterial genera were mainly Firmicutes and Proteobacteria. 16S rRNA gene sequencing was employed for function prediction, and the results showed that the addition of penicillin G increased nitrification, reduced denitrification. The autoinducer-1 (AI-1), autoinducer-3 (AI-3) and Phr signal molecules further participated in the nitrogen cycle by regulating the population behavior among multiple bacterial genera. In addition, SEM analysis showed that the quorum sensing system negatively regulated the abundance of genus related to the nitrogen conversion during chicken manure aerobic composting. This is a new theoretical analysis of the research on the treatment of hazardous materials.
本研究探讨了不同浓度青霉素 G 对鸡粪好氧堆肥过程中氮转化、细菌群落组成和群体感应的影响。堆肥后，添加青霉素 G 下调了 71 个属的丰度，上调了 103 个属的丰度。这些细菌属主要是厚壁菌门和变形菌门。16S rRNA 基因测序用于功能预测，结果表明添加青霉素 G 增加了硝化作用，减少了反硝化作用。自诱导物-1（AI-1）、自诱导物-3（AI-3）和 Phr 信号分子通过调节多种细菌属之间的种群行为进一步参与氮循环。此外，SEM 分析表明，群体感应系统负调控与鸡粪好氧堆肥中氮转化相关属的丰度。这是对危险物质处理研究的新理论分析。
