Laboratory of Biomass and Bioprocessing Engineering, College of Engineering, China Agricultural University, Beijing, 100083, China.
Laboratory of Biomass and Bioprocessing Engineering, College of Engineering, China Agricultural University, Beijing, 100083, China.
J Hazard Mater. 2019 Jan 15;362:258-265. doi: 10.1016/j.jhazmat.2018.09.028. Epub 2018 Sep 11.
This study systematically analyzed the effects of amoxicillin (AMX) on the nitrogen transformation and its corresponding functional bacterial communities by conducting two aerobic composting experiments, and AMX impact on bacterial community succession was also evaluated. It provides theoretical and methodological support for harmless composting treatment of large quantities of manure containing AMX in China and for the high-quality compost products. The results showed that AMX exerted several effects on basic physicochemical and biological compost parameters. Notably, temperature changes typically accompanying compost maturation were delayed in AMX compost, reflecting altered compost maturation kinetics and bacterial community structure. Moreover, relative to control, AMX inhibited growth and reproduction of dominant bacterial phyla Firmicutes and Bacteroidetes, with respective reductions of 17.8-26.1% and 0-7.76% in relative abundance (RA) and significantly increased Proteobacteria RA by 1.9-24.8%. Thus, AMX altered both compost bacterial community structure and succession. From the perspective of various nitrogen content changes, AMX has a significant effect on nitrogen conversion and release. Simultaneously, AMX may inhibit ammoniated and ammonia-oxidizing bacterial activity, while significantly increasing the RA of denitrifying bacteria. Indeed, during early composting with AMX, the RA of denitrifying bacteria was 1361.9-1435.0% of control, highlighting differences in nitrogen transformation and release between groups.
本研究通过开展两项有氧堆肥实验，系统分析了阿莫西林（AMX）对氮转化及其相应功能细菌群落的影响，并评估了 AMX 对细菌群落演替的影响。为我国大量含 AMX 粪便进行无害化堆肥处理和生产高质量堆肥产品提供了理论和方法学支持。结果表明，AMX 对基本理化和生物堆肥参数产生了多种影响。值得注意的是，AMX 堆肥中通常伴随堆肥成熟而发生的温度变化被延迟，反映出堆肥成熟动力学和细菌群落结构发生了改变。此外，与对照相比，AMX 抑制了优势细菌门厚壁菌门和拟杆菌门的生长和繁殖，相对丰度（RA）分别降低了 17.8-26.1%和 0-7.76%，而变形菌门的 RA 则显著增加了 1.9-24.8%。因此，AMX 改变了堆肥细菌群落结构和演替。从各种氮含量变化的角度来看，AMX 对氮转化和释放有显著影响。同时，AMX 可能抑制氨化和氨氧化细菌的活性，同时显著增加反硝化细菌的 RA。事实上，在 AMX 早期堆肥过程中，反硝化细菌的 RA 是对照的 1361.9-1435.0%，这突出了两组之间氮转化和释放的差异。
