Inamizu T, Kinohara N, Chang M P, Makinodan T
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2488-91. doi: 10.1073/pnas.83.8.2488.
The frequency of clonable 6-thioguanine-resistant (6-TGr) splenic T cells increased moderately with age in female BALB/c mice ranging in age from 3 to 32 months; however, the correlation between the frequency of clonable 6-TGr cells and age was weak. Those clonable 6-TGr T cells were deficient in hypoxanthine/guanine phosphoribosyltransferase (HGPRT) activity and sensitive to hypoxanthine/aminopterin/thymidine medium, as in the case of HGPRT-deficient L5178Y mouse lymphoma cells. When splenic T cells of individual aging mice were assessed simultaneously for the frequency of clonable 6-TGr T cells and for their ability to produce interleukin 2 or to proliferate in response to mitogenic stimulation, an inverse correlation was observed. These results indicate that the frequency of 6-TGr T cells is more closely related to physiologic age than chronologic age. This would mean that the frequency could be used as an index of physiologic age and that the T cells could serve as a cellular model relating gene alterations to physiologic age.
在3至32月龄的雌性BALB/c小鼠中，可克隆的6-硫鸟嘌呤抗性（6-TGr）脾T细胞的频率随年龄适度增加；然而，可克隆的6-TGr细胞频率与年龄之间的相关性较弱。这些可克隆的6-TGr T细胞缺乏次黄嘌呤/鸟嘌呤磷酸核糖转移酶（HGPRT）活性，并且对次黄嘌呤/氨基蝶呤/胸腺嘧啶核苷培养基敏感，这与HGPRT缺陷的L5178Y小鼠淋巴瘤细胞的情况相同。当同时评估个体衰老小鼠脾T细胞中可克隆的6-TGr T细胞的频率及其产生白细胞介素2或响应有丝分裂刺激而增殖的能力时，观察到负相关。这些结果表明，6-TGr T细胞的频率与生理年龄比与实际年龄更密切相关。这意味着该频率可作为生理年龄的指标，并且T细胞可作为将基因改变与生理年龄相关联的细胞模型。
