Pan Jason, Kmieciak Tommy, Liu Yen-Ting, Wildenradt Matthew, Chen Yun-Sheng, Zhao Yang
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States of America.
Department of Engineering Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States of America.
J Phys D Appl Phys. 2021 Dec 2;54(48). doi: 10.1088/1361-6463/ac2170. Epub 2021 Sep 9.
Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.
机械信号被认为在细胞功能和细胞命运决定中发挥着重要作用，然而，要在活细胞中以单分子灵敏度和分辨率直接测量这些物理量具有挑战性。在本综述中，我们重点关注两种有望在单分子水平探测力的主要技术。我们回顾了它们的理论基础、最近的技术进展以及未来方向，这些都是专门针对在活细胞中研究机械敏感分子而定制的。
