Khan Tawseef, Nasreen Nasreen, Shater Abdullah F, Khan Wali, Khan Adil, Kamal Mustafa, Vinueza Rommel, Leon Renato, Alhimaidi Ahmad R, Al-Jabr Omar A
Department of Zoology, Abdul Wali Khan University Mardan, 23200, Pakistan.
Department of Medical Laboratory Technology , Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia.
Saudi J Biol Sci. 2021 Dec;28(12):7022-7026. doi: 10.1016/j.sjbs.2021.07.078. Epub 2021 Aug 2.
The present study was designed to investigate the prevalence of gastrointestinal (GI) parasites in cattle and buffaloes of Lower Dir Khyber Pakhtunkhwa, Pakistan. The presence of the eggs, cysts, and oocysts of GI parasites in fecal samples were detected using direct smear methods and concentration techniques including floatation, centrifugation, and sedimentation. Identification of recovered fecal stages were determined by morphology using size and appearance of the recovered eggs, cysts, and oocysts. A total of 314 fecal samples were collected from the different Tehsils (Administrative Districts) and analyzed through microscopy. A higher prevalence was observed in the buffalo than the cow population. A total of 184 samples were positive for GI parasites of which 109/196 (55.61%) were from cattle, whereas 75/118 (63.55%) were from buffaloes. The minimum number of strongyle eggs detected in all the samples were 136.39 eggs/g (EPG). The mean EPG in cattle was 143.30 and 122.56 in buffaloes. The open-source water prevalence of GI parasites was higher than the other sources in cattle and the second highest after tap water in buffaloes. The seasonal prevalence of GI parasites ranged from 32.39% (23/71), in spring to 68.8% (86/125) in summer in cattle. In was For buffaloes the infection prevalence was 52.94% (27/51) and 71.64% (48/67) in spring and summer, respectively. Gastrointestinal parasites are a serious problem in cattle and buffaloes in the lower district of Dir Khyber Pakhtunkhwa Pakistan. In general, the burden of parasitic infection was low in most animals that received previous anti-parasitic treatment.
本研究旨在调查巴基斯坦开伯尔-普赫图赫瓦省下迪尔地区牛和水牛胃肠道（GI）寄生虫的流行情况。采用直接涂片法以及包括漂浮法、离心法和沉淀法在内的浓缩技术，检测粪便样本中胃肠道寄生虫的虫卵、包囊和卵囊。通过观察回收的虫卵、包囊和卵囊的大小及外观，利用形态学方法确定回收的粪便阶段。总共从不同的乡（行政区）收集了314份粪便样本，并通过显微镜进行分析。观察到水牛的感染率高于牛群。共有184份样本检测出胃肠道寄生虫呈阳性，其中109/196（55.61%）来自牛，而75/118（63.55%）来自水牛。所有样本中检测到的最小圆线虫卵数量为136.39个卵/克（EPG）。牛的平均EPG为143.30，水牛为122.56。在牛中，使用开源水源的胃肠道寄生虫感染率高于其他水源，在水牛中仅次于自来水。胃肠道寄生虫的季节性感染率在牛群中，春季为32.39%（23/71），夏季为68.8%（86/125）。在水牛中，春季和夏季的感染率分别为52.94%（27/51）和71.64%（48/67）。在巴基斯坦开伯尔-普赫图赫瓦省迪尔地区下游，胃肠道寄生虫是牛和水牛面临的一个严重问题。总体而言，大多数接受过先前抗寄生虫治疗的动物的寄生虫感染负担较低。
