Guo Ying, Zhang Yating, Hou Ya, Guo Pengmei, Wang Xiaobo, Zhang Sanyin, Yang Peng
School of Basic Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Chengdu Fifth People's Hospital, Chengdu, China.
Front Pharmacol. 2021 Nov 8;12:749915. doi: 10.3389/fphar.2021.749915. eCollection 2021.
Danggui Buxue decoction (DBD), consisting of Angelicae Sinensis Radix (ASR) and Astragali Radix (AR), is a famous prescription with the function of antivasoconstriction. This study intends to probe its mechanisms on the relaxation of the middle cerebral artery (MCA). Vascular tension of rat MCA was measured using a DMT620 M system. First, the identical series of concentrations of DBD, ASR, and AR were added into resting KCl and U46619 preconstricted MCA. According to the compatibility ratio, their dilatation effects were further investigated on KCl and U46619 preconstricted vessels. Third, four K channel blockers were employed to probe the vasodilator mechanism on KCl-contracted MCA. We finally examined the effects of DBD, ASR, and AR on the vascular tone of U46619-contracted MCA in the presence or absence of Ca. Data suggested that DBD, ASR, and AR can relax on KCl and U46619 precontracted MCA with no effects on resting vessels. The vasodilator effect of ASR was greater than those of DBD and AR on KCl-contracted MCA. For U46619-contracted MCA, ASR showed a stronger vasodilator effect than DBD and AR at low concentrations, but DBD was stronger than ASR at high concentrations. Amazingly, the vasodilator effect of DBD was stronger than that of AR at all concentrations on two vasoconstrictors which evoked MCA. The vasodilator effect of ASR was superior to that of DBD at a compatibility ratio on KCl-contracted MCA at low concentrations, while being inferior to DBD at high concentrations. However, DBD exceeded AR in vasodilating MCA at all concentrations. For U46619-constricted MCA, DBD, ASR, and AR had almost identical vasodilation. The dilation of DBD and AR on KCl-contracted MCA was independent of K channel blockers. However, ASR may inhibit the K channel opening partially through synergistic interactions with Gli and BaCl. DBD, ASR, and AR may be responsible for inhibiting [Ca], while ASR and AR can also inhibit [Ca]. DBD can relax MCA with no effects on resting vessels. The mechanism may be related to ASR's inhibition of K and K channels. Meanwhile, the inhibition of [Ca] by DBD, ASR, and AR as well as the inhibition of [Ca] by ASR and AR may contribute to dilate MCA.
当归补血汤（DBD）由当归（ASR）和黄芪（AR）组成，是一种具有抗血管收缩作用的著名方剂。本研究旨在探讨其舒张大脑中动脉（MCA）的机制。使用DMT620 M系统测量大鼠MCA的血管张力。首先，将相同系列浓度的DBD、ASR和AR添加到静息KCl和U46619预收缩的MCA中。根据配伍比例，进一步研究它们对KCl和U46619预收缩血管的舒张作用。第三，使用四种钾通道阻滞剂探讨对KCl收缩的MCA的血管舒张机制。我们最后研究了在有或无钙的情况下DBD、ASR和AR对U46619收缩的MCA血管张力的影响。数据表明，DBD、ASR和AR可舒张KCl和U46619预收缩的MCA，对静息血管无影响。ASR对KCl收缩的MCA的血管舒张作用大于DBD和AR。对于U46619收缩的MCA，ASR在低浓度时比DBD和AR表现出更强的血管舒张作用，但在高浓度时DBD比ASR更强。令人惊讶的是，在引起MCA收缩的两种血管收缩剂作用下，DBD在所有浓度下的血管舒张作用均强于AR。在低浓度时，ASR在KCl收缩的MCA上按配伍比例的血管舒张作用优于DBD，而在高浓度时则不如DBD。然而，在所有浓度下DBD舒张MCA的作用均超过AR。对于U46619收缩的MCA，DBD、ASR和AR的血管舒张作用几乎相同。DBD和AR对KCl收缩的MCA的舒张作用与钾通道阻滞剂无关。然而，ASR可能通过与Gli和BaCl的协同相互作用部分抑制钾通道开放。DBD、ASR和AR可能负责抑制[Ca]，而ASR和AR也可抑制[Ca]。DBD可舒张MCA，对静息血管无影响。其机制可能与ASR对钾通道和钾通道的抑制有关。同时，DBD、ASR和AR对[Ca]的抑制以及ASR和AR对[Ca]的抑制可能有助于舒张MCA。
