King G J, Huang W M
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7248-52. doi: 10.1073/pnas.79.23.7248.
Two physical origins of T4 DNA replication were determined by hybridization of viral DNA prepared 2.5 min after infection to a display of total T4 DNA. This is the earliest time after T4 infection of Escherichia coli at 37 degrees C that labeled and hybridizable DNA can be detected. The two origins, separated by about 25 kilobases, were identified and localized in the early region of the T4 map. One of them is located in a 5.6-kilobase EcoRI fragment containing genes 62-46. The other is located between genes rI and e in a 1.9-kilobase EcoRI fragment. Both of these T4 fragments have been cloned and their interactions with the host cell are discussed.
通过将感染后2.5分钟制备的病毒DNA与完整的T4 DNA杂交，确定了T4 DNA复制的两个物理起源。这是在37摄氏度下T4感染大肠杆菌后最早能检测到标记且可杂交DNA的时间。这两个起源相隔约25千碱基，在T4图谱的早期区域被识别并定位。其中一个位于一个包含基因62 - 46的5.6千碱基的EcoRI片段中。另一个位于基因rI和e之间的一个1.9千碱基的EcoRI片段中。这两个T4片段都已被克隆，并讨论了它们与宿主细胞的相互作用。
