从大肠杆菌中分离膜相关折叠染色体：蛋白质合成抑制的影响
Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition.
作者信息
Ryder O A, Smith D W
出版信息
J Bacteriol. 1974 Dec;120(3):1356-63. doi: 10.1128/jb.120.3.1356-1363.1974.
Abstract
The sedimentation properties of membrane-associated folded chromosomes prepared from Escherichia coli TAU-bar at 0 to 4 C were studied. Utilizing a modification of the procedure of Stonington and Pettijohn (1971), quantitative yields of membrane-associated folded chromosomes may be obtained. Folded chromosomes remained associated with the cell envelope during their replication and after completion of residual synthesis in the absence of required amino acids, as demonstrated by sedimentation velocities and the presence of high levels of cosedimenting protein. Membrane-associated folded chromosomes isolated from amino acid-starved cells sedimented more rapidly than membrane-associated folded chromosomes isolated from exponentially growing cells.
摘要
研究了在0至4摄氏度下从大肠杆菌TAU-bar制备的膜相关折叠染色体的沉降特性。利用对斯托宁顿和佩蒂约翰（1971年）方法的改进，可以获得膜相关折叠染色体的定量产量。如沉降速度和高水平共沉降蛋白的存在所表明的，折叠染色体在其复制过程中以及在缺乏必需氨基酸时残留合成完成后仍与细胞膜结合。从氨基酸饥饿细胞中分离出的膜相关折叠染色体比从指数生长细胞中分离出的膜相关折叠染色体沉降得更快。
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