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1
Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition.从大肠杆菌中分离膜相关折叠染色体：蛋白质合成抑制的影响
J Bacteriol. 1974 Dec;120(3):1356-63. doi: 10.1128/jb.120.3.1356-1363.1974.
2
Properties of membrane-associated folded chromosomes of E. coli related to initiation and termination of DNA replication.大肠杆菌膜相关折叠染色体与DNA复制起始和终止相关的特性
Cell. 1975 Apr;4(4):337-45. doi: 10.1016/0092-8674(75)90154-3.
3
Properties of a membrane-attached form of the folded chromosome of Escherichia coli.大肠杆菌折叠染色体膜附着形式的特性
J Mol Biol. 1974 Jan 5;82(1):91-105. doi: 10.1016/0022-2836(74)90576-2.
4
On the structure of the folded chromosome of Escherichia coli.关于大肠杆菌折叠染色体的结构
J Mol Biol. 1972 Nov 14;71(2):127-47. doi: 10.1016/0022-2836(72)90342-7.
5
Membrane attachment of folded chromosome of Escherichia coli.大肠杆菌折叠染色体的膜附着
Biochem J. 1976 Jan 15;154(1):239-41. doi: 10.1042/bj1540239.
6
Factors affecting the release of folded chromosomes from Escherichia coli.影响大肠杆菌中折叠染色体释放的因素。
Eur J Biochem. 1976 Apr 1;63(2):469-75. doi: 10.1111/j.1432-1033.1976.tb10249.x.
7
Significance of folded chromosomes released from amino-acid-starved Escherichia coli cells.从氨基酸饥饿的大肠杆菌细胞中释放出的折叠染色体的意义。
Eur J Biochem. 1976 Jun 1;65(2):409-14. doi: 10.1111/j.1432-1033.1976.tb10355.x.
8
Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.大肠杆菌的包膜相关折叠染色体：不同生理条件下的变化
J Bacteriol. 1976 Aug;127(2):904-16. doi: 10.1128/jb.127.2.904-916.1976.
9
Accumulation of replicative intermediates and catenated forms of the colicinogenic factor E 1 in E. coli during the replication at elevated temperatures.在高温下复制过程中，大肠杆菌中致育因子E1的复制中间体和连环形式的积累。
Biochem Biophys Res Commun. 1972 Dec 18;49(6):1699-708. doi: 10.1016/0006-291x(72)90539-6.
10
Protein synthesis and the release of the replicated chromosome from the cell membrane.蛋白质合成以及复制后的染色体从细胞膜上释放。
Nature. 1974 Sep 20;251(5472):252-4. doi: 10.1038/251252a0.

引用本文的文献

1
Determination of molecular weights by fluctuation spectroscopy: application to DNA.通过涨落光谱法测定分子量：应用于DNA
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2776-80. doi: 10.1073/pnas.73.8.2776.
2
Electron microscopy of membrane-free folded chromosomes from Escherichia coli.大肠杆菌无膜折叠染色体的电子显微镜观察。
Chromosoma. 1976 Dec 16;59(2):89-101. doi: 10.1007/BF00328479.
3
Properties of condensed bacteriophage T4 DNA isolated from Escherichia coli infected with bacteriophage T4.从感染了噬菌体T4的大肠杆菌中分离出的浓缩噬菌体T4 DNA的特性。
J Virol. 1976 Sep;19(3):1012-27. doi: 10.1128/JVI.19.3.1012-1027.1976.
4
Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.大肠杆菌的包膜相关折叠染色体：不同生理条件下的变化
J Bacteriol. 1976 Aug;127(2):904-16. doi: 10.1128/jb.127.2.904-916.1976.
5
Nonintegrated plasmid-chromosome complexes in Escherichia coli.大肠杆菌中的非整合质粒 - 染色体复合体
J Bacteriol. 1976 Aug;127(2):881-9. doi: 10.1128/jb.127.2.881-889.1976.
6
Studies of DNA bound RNA molecules isolated from nucleoids of Escherichia coli.对从大肠杆菌类核中分离出的与DNA结合的RNA分子的研究。
Nucleic Acids Res. 1976 Mar;3(3):767-88. doi: 10.1093/nar/3.3.767.
7
Comparative studies on membrane-associated, folded chromosomes from Escherichia coli.大肠杆菌膜相关折叠染色体的比较研究。
J Bacteriol. 1976 Apr;126(1):64-71. doi: 10.1128/jb.126.1.64-71.1976.
8
Electron microscopy of membrane-associated folded chromosomes of Escherichia coli.大肠杆菌膜相关折叠染色体的电子显微镜观察
Chromosoma. 1976 Mar 31;55(1):13-25. doi: 10.1007/BF00288323.
9
Shutoff of host macromolecular synthesis after T-even bacteriophage infection.T-偶数噬菌体感染后宿主大分子合成的关闭。
Microbiol Rev. 1979 Jun;43(2):199-223. doi: 10.1128/mr.43.2.199-223.1979.
10
Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: sedimentation properties of irradiated nucleoids and chromosomal deoxyribonucleic acid.电离辐射对大肠杆菌K-12折叠染色体的损伤：辐照类核及染色体脱氧核糖核酸的沉降特性
J Bacteriol. 1979 May;138(2):475-85. doi: 10.1128/jb.138.2.475-485.1979.

本文引用的文献

1
EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI.大肠杆菌中调控染色体复制机制的两个不同方面的证据。
J Mol Biol. 1964 Oct;10:120-36. doi: 10.1016/s0022-2836(64)80032-2.
2
SEDIMENTATION AND BIOLOGICAL PROPERTIES OF T-PHAGES OF ESCHERICHIA COLI.大肠杆菌T噬菌体的沉降及生物学特性
Virology. 1964 Jul;23:408-18. doi: 10.1016/0042-6822(64)90264-8.
3
THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION.氨基酸剥夺对后续脱氧核糖核酸复制的影响。
Biochim Biophys Acta. 1963 Sep 17;76:9-24.
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The replication of bacteriophage MS2. 1. Transfer of parental nucleic acid to progeny phage.噬菌体MS2的复制。1. 亲代核酸向子代噬菌体的转移。
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Involvement of synthesis of RNA in thymineless death.RNA合成参与无胸腺嘧啶死亡过程。
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Thymine deficiency and the normal DNA replication cycle. I.胸腺嘧啶缺乏与正常DNA复制周期。I.
J Mol Biol. 1961 Apr;3:144-55. doi: 10.1016/s0022-2836(61)80041-7.
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Electron microscopy of chloramphenicol-treated Escherichia coli.氯霉素处理的大肠杆菌的电子显微镜检查。
J Bacteriol. 1967 Jun;93(6):1987-2002. doi: 10.1128/jb.93.6.1987-2002.1967.
8
Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication.甲硫氨酸及其他氨基酸饥饿对后续细菌脱氧核糖核酸复制的影响。
J Bacteriol. 1966 Sep;92(3):609-17. doi: 10.1128/jb.92.3.609-617.1966.
9
Escherichia coli mutants temperature-sensitive for DNA synthesis.对DNA合成温度敏感的大肠杆菌突变体。
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
10
Escherichia coli mutants with temperature-sensitive synthesis of DNA.具有温度敏感型DNA合成能力的大肠杆菌突变体。
Mol Gen Genet. 1970;109(2):107-22. doi: 10.1007/BF00269647.

从大肠杆菌中分离膜相关折叠染色体：蛋白质合成抑制的影响

Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition.

作者信息

Ryder O A, Smith D W

出版信息

J Bacteriol. 1974 Dec;120(3):1356-63. doi: 10.1128/jb.120.3.1356-1363.1974.

DOI:10.1128/jb.120.3.1356-1363.1974
PMID:4612018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC245922/
Abstract

The sedimentation properties of membrane-associated folded chromosomes prepared from Escherichia coli TAU-bar at 0 to 4 C were studied. Utilizing a modification of the procedure of Stonington and Pettijohn (1971), quantitative yields of membrane-associated folded chromosomes may be obtained. Folded chromosomes remained associated with the cell envelope during their replication and after completion of residual synthesis in the absence of required amino acids, as demonstrated by sedimentation velocities and the presence of high levels of cosedimenting protein. Membrane-associated folded chromosomes isolated from amino acid-starved cells sedimented more rapidly than membrane-associated folded chromosomes isolated from exponentially growing cells.

摘要

研究了在0至4摄氏度下从大肠杆菌TAU-bar制备的膜相关折叠染色体的沉降特性。利用对斯托宁顿和佩蒂约翰（1971年）方法的改进，可以获得膜相关折叠染色体的定量产量。如沉降速度和高水平共沉降蛋白的存在所表明的，折叠染色体在其复制过程中以及在缺乏必需氨基酸时残留合成完成后仍与细胞膜结合。从氨基酸饥饿细胞中分离出的膜相关折叠染色体比从指数生长细胞中分离出的膜相关折叠染色体沉降得更快。