Toral Laura, Rodríguez Miguel, Martínez-Checa Fernando, Montaño Alfredo, Cortés-Delgado Amparo, Smolinska Agnieszka, Llamas Inmaculada, Sampedro Inmaculada
Xtrem Biotech S.L., European Business Innovation Center, Avenida de la Innovación, Granada, Spain.
Department of Microbiology, Faculty of Pharmacy, Campus de Cartuja s/n, Granada, Spain.
Front Microbiol. 2021 Nov 12;12:773092. doi: 10.3389/fmicb.2021.773092. eCollection 2021.
Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20-25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera , , , and to inhibit the growth of , , , , , and , and . We analysed bacterial VOCs by using GC/MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. growth inhibition assays and experiments using cherry fruits showed that the best producers of VOCs, L193, XT1 and Z8, exhibited the highest antifungal activity against , and , which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death.
采后果蔬中的植物病原真菌造成了20%-25%的产量损失。挥发性有机化合物（VOCs）作为一种安全且环保的替代农药，在食品工业中对于对抗这些植物病原真菌正变得越来越重要。在本研究中，我们分析了由 属、 属、 属、 属和 属菌株产生的一些挥发性有机化合物抑制 、 、 、 、 、 和 生长的能力。我们通过气相色谱/质谱联用仪（GC/MS）分析细菌挥发性有机化合物，并鉴定出87种挥发性化合物，特别是3-羟基丁酮、乙酸、2,3-丁二醇、异戊醇、二甲基二硫和异戊基异丁酸酯。使用樱桃果实进行的生长抑制试验和 实验表明，挥发性有机化合物的最佳产生菌，即 L193、 XT1和 Z8，对 、 和 表现出最高的抗真菌活性，这突出了这些菌株控制采后病害的潜力。经细菌挥发性有机化合物处理的真菌的透射电子显微镜照片清楚地显示出抗真菌活性，这导致菌丝体的细胞成分严重退化和细胞死亡。
