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葡萄基因过表达增强烟草的耐盐性。

Overexpression of Grapevine Gene Enhanced Salt Tolerance in Tobacco.

机构信息

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu, China.

出版信息

Int J Mol Sci. 2020 Feb 15;21(4):1323. doi: 10.3390/ijms21041323.

DOI:10.3390/ijms21041323
PMID:32075333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7072961/
Abstract

In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators that regulate developmental process and responses to phytohormones and stress treatments. However, the regulatory functions of the L. (grapevine) Aux/IAA transcription factor gene have not been reported. In this study, the gene was successfully cloned from grapevine. Subcellular localization analysis in onion epidermal cells indicated that VvIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length of exhibited transcriptional activation. Salt tolerance in transgenic tobacco plants and was significantly enhanced by overexpression. Real-time quantitative PCR analysis showed that overexpression of up-regulated the salt stress-responsive genes, including pyrroline-5-carboxylate synthase (), late embryogenesis abundant protein (), superoxide dismutase (), and peroxidase () genes, under salt stress. Enzymatic analyses found that the transgenic plants had higher SOD and POD activities under salt stress. Meanwhile, component analysis showed that the content of proline in transgenic plants increased significantly, while the content of hydrogen peroxide (HO) and malondialdehyde (MDA) decreased significantly. Based on the above results, the gene is related to improving the salt tolerance of transgenic tobacco plants. The gene has the potential to be applied to enhance plant tolerance to abiotic stress.

摘要

在植物中，生长素/吲哚乙酸（Aux/IAA）蛋白是转录调节剂，可调节发育过程以及对植物激素和胁迫处理的反应。然而，尚未报道 L.（葡萄）Aux/IAA 转录因子基因的调节功能。在本研究中，成功地从葡萄中克隆了该基因。在洋葱表皮细胞中的亚细胞定位分析表明 VvIAA18 定位于细胞核。酵母中的表达分析表明全长表现出转录激活。通过过表达，转基因烟草植物和的耐盐性显著增强。实时定量 PCR 分析显示，过表达上调了盐胁迫响应基因，包括吡咯啉-5-羧酸合酶（）、晚期胚胎丰富蛋白（）、超氧化物歧化酶（）和过氧化物酶（）基因，在盐胁迫下。酶分析发现，转基因植物在盐胁迫下具有更高的 SOD 和 POD 活性。同时，成分分析表明，转基因植物中的脯氨酸含量显著增加，而过氧化氢（HO）和丙二醛（MDA）的含量显著降低。基于上述结果，该基因与提高转基因烟草植物的耐盐性有关。该基因有可能被应用于增强植物对非生物胁迫的耐受性。

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