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硫辛酸生物合成的表征：线粒体辛酰转移酶和硫辛酰合成酶酶系统

Characterization of Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System.

作者信息

Martins-Noguerol Raquel, Acket Sébastien, Troncoso-Ponce M Adrián, Garcés Rafael, Thomasset Brigitte, Venegas-Calerón Mónica, Salas Joaquín J, Martínez-Force Enrique, Moreno-Pérez Antonio J

机构信息

Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Seville, Spain.

UPJV, UMR CNRS 7025, Enzyme and Cell Engineering, Centre de Recherche Royallieu, Université de Technologie de Compiègne, Compiègne, France.

出版信息

Front Plant Sci. 2021 Nov 18;12:781917. doi: 10.3389/fpls.2021.781917. eCollection 2021.

DOI:10.3389/fpls.2021.781917
PMID:34868183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8639206/
Abstract

Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower and genes ( and were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in and , where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained.

摘要

硫辛酸（LA，6,8 - 二硫辛酸）是一种含硫辅酶，对于大多数细菌和真核生物中参与氧化和一碳代谢的几种关键酶的活性至关重要。硫辛酸由辛酰转移酶（LIP2，EC 2.3.1.181）和硫辛酰胺合成酶（LIP1，EC 2.8.1.8）协同作用合成。在植物中，丙酮酸脱氢酶（PDH）、2 - 氧代戊二酸脱氢酶或甘氨酸脱羧酶是需要进行硫辛酰化的重要复合物。这些硫辛酰化的酶和复合物位于线粒体中，而PDH也存在于质体中，在那里它为脂肪酸生物合成提供乙酰辅酶A。因此，PDH的硫辛酰化可能在这两个细胞器中调节脂肪酸合成。在本研究中，分离、测序、克隆并鉴定了向日葵的 和 基因，评估了它们假定的线粒体定位。研究了这些基因在不同组织中的表达，并对蛋白质对接进行了建模。这些基因也在 和 中表达，评估了它们对脂肪酸和甘油脂质组成的影响。拟南芥的脂质组学研究揭示了过表达这些酶的株系中的脂质重塑，并根据所得结果讨论了两种向日葵蛋白在观察到的表型中的作用。

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