Sanya Nanfan Research Institution of Hainan University, Sanya 572025, China.
Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Int J Mol Sci. 2023 Sep 13;24(18):14039. doi: 10.3390/ijms241814039.
MIKC-type MADS-box genes, also known as type II genes, play a crucial role in regulating the formation of floral organs and reproductive development in plants. However, the genome-wide identification and characterization of type II genes as well as a transcriptomic survey of their potential roles in remain unresolved. Here, we identified and characterized 24 type II genes in the genome, and investigated their evolutional scenario and potential roles with a widespread expression profile. The type II genes were divided into thirteen subclades, and gene loss events likely occurred in papaya, as evidenced by the contracted member size of most subclades. Gene duplication mainly contributed to MIKC-type gene formation in papaya, and the duplicated gene pairs displayed prevalent expression divergence, implying the evolutionary significance of gene duplication in shaping the diversity of type II genes in papaya. A large-scale transcriptome analysis of 152 samples indicated that different subclasses of these genes showed distinct expression patterns in various tissues, biotic stress response, and abiotic stress response, reflecting their divergent functions. The hub-network of male and female flowers and qRT-PCR suggested that and participated in male flower development and seed germination. Overall, this study provides valuable insights into the evolution and functions of MIKC-type genes in .
MIKC 型 MADS 框基因，也称为 II 型基因，在植物花器官的形成和生殖发育的调控中起着至关重要的作用。然而，II 型基因的全基因组鉴定和特征，以及它们在 中的潜在作用的转录组调查仍未解决。在这里，我们在 基因组中鉴定和表征了 24 个 II 型基因，并通过广泛的表达谱研究了它们的进化情景和潜在作用。II 型基因被分为十三个亚科，基因丢失事件可能发生在木瓜中，这表明大多数亚科的成员大小收缩。基因复制主要导致了木瓜中 MIKC 型基因的形成，而复制的基因对表现出普遍的表达分歧，这表明基因复制在塑造木瓜中 II 型基因多样性方面具有进化意义。对 152 个样本的大规模转录组分析表明，这些基因的不同亚类在不同组织、生物胁迫反应和非生物胁迫反应中表现出不同的表达模式，反映了它们的功能分化。雄花和雌花的枢纽网络以及 qRT-PCR 表明 和 参与了雄花发育和种子萌发。总的来说，这项研究为 MIKC 型基因在 中的进化和功能提供了有价值的见解。
