Yan Jinhua, Yao Ling, Li Ping, Wu Guohe, Lv Xiaobin
Department of Hematology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
Department of Gastroenterology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
Oncol Lett. 2022 Jan;23(1):24. doi: 10.3892/ol.2021.13142. Epub 2021 Nov 18.
Long non-coding (lnc)RNA MIR17HG has been identified as a oncogene whose roles in acute myeloid leukemia (AML) remain unclear. The present study aimed to investigate the role of lncRNA MIR17HG in AML. Differential expression of MIR17HG in AML was determined by reverse transcription-quantitative PCR. Overexpression assays and dual luciferase reporter assays were performed to determine the relationship between MIR17HG and microRNA (miR)-21, and apoptosis was analyzed by using an apoptosis assay. The results showed that the expression of MIR17HG was decreased in AML, which was further decreased following homoharringtonine (HHT)-based chemotherapy. Bioinformatics analysis predicted that miR-21 could bind with MIR17HG. However, miR-21 overexpression had no effect on the expression level of MIR17HG. Dual luciferase reporter assays were performed to verify the direct interaction between miR-21 and MIR17HG. In addition, overexpression of MIR17HG and miR-21 in AML cell lines up- and downregulated the expression level of PTEN, respectively. Furthermore, cell apoptosis showed that MIR17HG and PTEN overexpression enhanced cell apoptosis following cell treatment with HTT. However, miR-21 overexpression exerted the opposite effect, since it reversed the effects of MIR17HG and PTEN overexpression in AML cell apoptosis. In conclusion, the current study suggested that MIR17HG could regulate the miR-21/PTEN axis to modulate the chemoresistance of AML cells.
长链非编码（lnc）RNA MIR17HG已被鉴定为一种癌基因，其在急性髓系白血病（AML）中的作用尚不清楚。本研究旨在探讨lncRNA MIR17HG在AML中的作用。通过逆转录定量PCR测定AML中MIR17HG的差异表达。进行过表达实验和双荧光素酶报告基因实验以确定MIR17HG与微小RNA（miR）-21之间的关系，并使用凋亡检测分析细胞凋亡情况。结果显示，AML中MIR17HG的表达降低，基于高三尖杉酯碱（HHT）的化疗后其表达进一步降低。生物信息学分析预测miR-21可与MIR17HG结合。然而，miR-21过表达对MIR17HG的表达水平没有影响。进行双荧光素酶报告基因实验以验证miR-21与MIR17HG之间的直接相互作用。此外，AML细胞系中MIR17HG和miR-21的过表达分别上调和下调了PTEN的表达水平。此外，细胞凋亡实验表明，MIR17HG和PTEN过表达增强了HTT处理后细胞的凋亡。然而，miR-21过表达则产生相反的效果，因为它逆转了MIR17HG和PTEN过表达对AML细胞凋亡的影响。总之，当前研究表明MIR17HG可调节miR-21/PTEN轴以调节AML细胞的化疗耐药性。
