基于水通道蛋白4-IgG活细胞检测在视神经脊髓炎谱系障碍中的诊断价值。
Diagnostic value of aquaporin-4-IgG live cell based assay in neuromyelitis optica spectrum disorders.
作者信息
Redenbaugh Vyanka, Montalvo Mayra, Sechi Elia, Buciuc Marina, Fryer James P, McKeon Andrew, Lennon Vanda A, Mills John R, Weinshenker Brian G, Wingerchuk Dean M, Chen John J, Tariq Bhatti M, Lopez Chiriboga A Sebastian, Pittock Sean J, Flanagan Eoin P
机构信息
Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA.
Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA.
出版信息
Mult Scler J Exp Transl Clin. 2021 Nov 26;7(4):20552173211052656. doi: 10.1177/20552173211052656. eCollection 2021 Oct.
OBJECTIVE
Determine the utility of aquaporin 4 IgG (AQP4-IgG) testing (live cell-based assay) for Neuromyelitis Optica Spectrum Disorders (NMOSD).
METHODS
We included Mayo Clinic patients (1/1/2018-12/31/2019) tested for serum AQP4-IgG by live cell-based flow-cytometric assay. Medical records were reviewed to assess if patients fulfilled 2015 NMOSD criteria.
RESULTS
Of 1371 patients tested, 41 were positive (3%) and all fulfilled NMOSD criteria with AQP4-IgG (specificity = 100%). Only 10/1330 testing negative met NMOSD criteria without AQP4-IgG (sensitivity = 80%) and seven of these 10 were MOG-IgG positive.
CONCLUSIONS
AQP4-IgG by live cell-based assay was highly specific and without false positives in a high throughput setting.
目的
确定水通道蛋白4免疫球蛋白（AQP4-IgG）检测（基于活细胞的检测方法）对视神经脊髓炎谱系障碍（NMOSD）的实用性。
方法
我们纳入了梅奥诊所2018年1月1日至2019年12月31日期间通过基于活细胞的流式细胞术检测血清AQP4-IgG的患者。查阅病历以评估患者是否符合2015年NMOSD标准。
结果
在1371例接受检测的患者中，41例呈阳性（3%），所有这些患者均符合AQP4-IgG阳性的NMOSD标准（特异性 = 100%）。在1330例检测结果为阴性的患者中，只有10例在无AQP4-IgG的情况下符合NMOSD标准（敏感性 = 80%），这10例患者中有7例MOG-IgG呈阳性。
结论
在高通量检测中，基于活细胞检测方法的AQP4-IgG具有高度特异性且无假阳性。
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