Li Tuanwei, Zhang Yejun, Wu Feng, Chen Guangcun, Li Chunyan, Wang Qiangbin
CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
Small Methods. 2025 Jan;9(1):e2400132. doi: 10.1002/smtd.202400132. Epub 2024 Mar 12.
Intravital fluorescence imaging in the second near-infrared window (NIR-II, 900-1700 nm) has emerged as a promising method for non-invasive diagnostics in complex biological systems due to its advantages of less background interference, high tissue penetration depth, high imaging contrast, and sensitivity. However, traditional NIR-II fluorescence imaging, which is characterized by the "always on" or "turn on" mode, lacks the ability of quantitative detection, leading to low reproducibility and reliability during bio-detection. In contrast, NIR-II ratiometric fluorescence imaging can realize quantitative and reliable analysis and detection in vivo by providing reference signals for fluorescence correction, generating new opportunities and prospects during in vivo bioimaging and biosensing. In this review, the current design strategies and sensing mechanisms of NIR-II ratiometric fluorescence probes for bioimaging and biosensing applications are systematically summarized. Further, current challenges, future perspectives and opportunities for designing NIR-II ratiometric fluorescence probes are also discussed. It is hoped that this review can provide effective guidance for the design of NIR-II ratiometric fluorescence probes and promote its adoption in reliable biological imaging and sensing in vivo.
由于具有背景干扰少、组织穿透深度高、成像对比度高和灵敏度高等优点，第二近红外窗口（NIR-II，900 - 1700 nm）的活体荧光成像已成为复杂生物系统中非侵入性诊断的一种有前途的方法。然而，传统的以“常开”或“开启”模式为特征的NIR-II荧光成像缺乏定量检测能力，导致生物检测过程中的重现性和可靠性较低。相比之下，NIR-II比率荧光成像可以通过提供荧光校正的参考信号，在体内实现定量和可靠的分析与检测，为体内生物成像和生物传感带来了新的机遇和前景。在这篇综述中，系统总结了用于生物成像和生物传感应用的NIR-II比率荧光探针的当前设计策略和传感机制。此外，还讨论了设计NIR-II比率荧光探针目前面临的挑战、未来展望和机遇。希望这篇综述能够为NIR-II比率荧光探针的设计提供有效指导，并促进其在可靠的体内生物成像和传感中的应用。
