Bol S J, Rosdorff H J, Ronteltap C P, Hennen L A
J Immunol Methods. 1986 Jun 10;90(1):15-23. doi: 10.1016/0022-1759(86)90378-9.
This study deals with the interpretation of primary data of the 51Cr release assay for cellular cytotoxicity. In particular the dose-effect relationship between increasing numbers of lymphoid cells and the percentage of target cells killed has been considered. The number of target cells killed depends on the frequency and the activity of cytotoxic cells. These two parameters are often not distinguished from each other, which causes confusion and frequently results in vague descriptions of cytotoxicity data. Because in many cases not all cells in the target cell population can be lysed, we recommend the introduction of the plateau value for target cell kill. This maximum of target cell kill is a measure of the frequency of lysable target cells, but also depends on the cytotoxic cell activity. Description of the dose-effect curve by y = A(1 - e-kx) allows the simultaneous calculation of the maximum kill (A) and the frequency of cytotoxic cells (k) in the lymphoid cell population (x). Results are presented which indicate that A and k represent totally independent biological parameters the use of which permits a more objective description of cytotoxicity data.
本研究涉及对用于细胞毒性的51Cr释放试验原始数据的解读。特别考虑了淋巴细胞数量增加与被杀伤靶细胞百分比之间的剂量效应关系。被杀伤的靶细胞数量取决于细胞毒性细胞的频率和活性。这两个参数常常未被区分开来，这会导致混淆并经常造成对细胞毒性数据的模糊描述。由于在许多情况下靶细胞群体中的所有细胞并非都能被裂解，我们建议引入靶细胞杀伤的平台值。这种靶细胞杀伤的最大值是可裂解靶细胞频率的一种度量，但也取决于细胞毒性细胞的活性。用y = A(1 - e-kx)描述剂量效应曲线，可同时计算淋巴细胞群体（x）中的最大杀伤值（A）和细胞毒性细胞的频率（k）。所呈现的结果表明，A和k代表完全独立的生物学参数，使用这些参数能够更客观地描述细胞毒性数据。
