Wang Wenqi, He Xiaowen, Du Mingzhi, Xie Chen, Zhou Wen, Huang Wei, Fan Quli
Key Laboratory for Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, China.
Frontiers Science Center for Flexible Electronics (FSCFE), MIIT Key Laboratory of Flexible Electronics (KLoFE), Northwestern Polytechnical University, Xi'an, China.
Front Chem. 2021 Nov 15;9:769655. doi: 10.3389/fchem.2021.769655. eCollection 2021.
Second near-infrared window (NIR-II) fluorescence imaging has shown great potential in the field of bioimaging. However, the excitation wavelengths of most NIR-II fluorescence dyes are in the first near-infrared (NIR-I) region, which leads to limited imaging depth and resolution. To address such issue, NIR-II fluorescence dyes with 1,064 nm excitation have been developed and applied for imaging. Compared with NIR-I wavelength excited dyes, 1,064 nm excited dyes exhibit a higher tissue penetration depth and resolution. The improved performance makes these dyes have much broader imaging applications. In this mini review, we summarize recent advances in 1,064 nm excited NIR-II fluorescence fluorophores for bioimaging. Two kinds of organic fluorophores, small molecule dye and semiconducting polymer (SP), are reviewed. The general properties of these fluorophores are first introduced. Small molecule dyes with different chemical structures for variety of bioimaging applications are then discussed, followed by the introduction of SPs for NIR-II phototheranostics. Finally, the conclusion and future perspective of this field is given.
第二近红外窗口（NIR-II）荧光成像在生物成像领域已显示出巨大潜力。然而，大多数NIR-II荧光染料的激发波长处于第一近红外（NIR-I）区域，这导致成像深度和分辨率受限。为解决这一问题，已开发出激发波长为1064 nm的NIR-II荧光染料并将其应用于成像。与NIR-I波长激发的染料相比，1064 nm激发的染料具有更高的组织穿透深度和分辨率。性能的提升使这些染料具有更广泛的成像应用。在本综述中，我们总结了用于生物成像的1064 nm激发的NIR-II荧光团的最新进展。综述了两种有机荧光团，即小分子染料和半导体聚合物（SP）。首先介绍了这些荧光团的一般特性。接着讨论了具有不同化学结构、用于各种生物成像应用的小分子染料，随后介绍了用于NIR-II光诊疗的半导体聚合物。最后给出了该领域的结论和未来展望。
