Department of Sustainability Sciences, El Colegio de la Frontera Sur Unidad Campeche, Av. Rancho Polígono 2-A Col. Ciudad Industrial, CP 24500, Lerma, Campeche, Campeche, Mexico.
Institute of Biotechnology, Department of Biological Sciences, Universidad Autónoma de Nuevo León, Av. Universidad s/n. Ciudad Universitaria, CP 66455, San Nicolás de los Garza, Nuevo León, Mexico.
Arch Microbiol. 2021 Dec 4;204(1):2. doi: 10.1007/s00203-021-02600-3.
To explore the capability of soil mycobiota to degrade avocado peel waste and identify relevant successions and trophic guild shifts, fungal communities from three environments with different land uses were evaluated in a solid-state process. Soil samples used as inoculum were collected from a pristine mature tropical forest, a traditionally managed Mayan land, and an intensively managed monospecific avocado plantation. Soil-substrate mixes were evaluated for 52 weeks to evaluate organic matter decay and the carbon-to-nitrogen ratio. Amplicon-based high-throughput sequencing from internally transcribed spacer (ITS) analysis revealed significant differences in fungal communities widely dominated by Fusarium sp. and Clonostachys sp.; however, less represented taxa showed relevant shifts concomitantly with organic matter content drops. Trophic guild assignment revealed different behaviors in fungal communities between treatments over the 52 weeks, suggesting distinct preconditioning of fungal communities in these environments. Overall, the results lead to the identification of promising degradation moments and inoculum sources for further consortia enrichment or bioprospecting efforts.
为了探索土壤真菌群落降解鳄梨皮废物的能力，并确定相关的演替和营养类群转变，本研究采用固态发酵法，评估了来自三种具有不同土地利用方式的环境中的真菌群落。用作接种物的土壤样本取自原始成熟的热带森林、传统管理的玛雅土地和集约化管理的单一鳄梨种植园。对土壤-基质混合物进行了 52 周的评估，以评估有机质的降解和碳氮比。基于内转录间隔区（ITS）分析的扩增子高通量测序显示，真菌群落存在显著差异，广泛由镰刀菌属（Fusarium sp.）和拟青霉属（Clonostachys sp.）主导；然而，较少代表的分类群也发生了相关的转变，伴随着有机质含量的下降。营养类群的分配揭示了不同处理之间真菌群落的不同行为，这表明这些环境中的真菌群落存在明显的预先条件。总的来说，这些结果确定了有希望的降解时刻和接种物来源，可用于进一步的群落富集或生物勘探工作。
