Sciuto Katia, Andreoli Carlo, Rascio Nicoletta, La Rocca Nicoletta, Moro Isabella
Department of Biology, University of Padova, via U. Bassi 58, B-35131 Padova, Italy.
Cladistics. 2012 Aug;28(4):357-374. doi: 10.1111/j.1096-0031.2011.00386.x. Epub 2011 Dec 20.
Cyanobacteria (phylum Cyanophyta/Cyanobacteria, class Cyanophyceae) are among the most widespread organisms and are able to adapt themselves to different extreme environments. These micro-organisms have an important ecological role, given their ability to perform oxygenic photosynthesis, and are employed in different fields based on their ability to produce several bioactive compounds. Their prokaryotic nature, the presence of many cryptic species, and the coexistence of different nomenclature systems make the taxonomic identification of cyanobacteria particularly difficult. Moreover, for several species, the original reference strains (holotypes) are lacking. Increasingly, authors are using a polyphasic approach to characterize cyanobacteria, while typification is a recent trend that is being used to solve the problem of missing holotypes in other micro-organisms. Here we focus on a filamentous cyanobacterium, isolated from the Euganean Thermal District (Padova, Italy) and temporarily named strain ETS-02, using a polyphasic approach that includes morphological, ultrastructural, biochemical (pigment and fatty acid content), physiological (nitrogen fixation), and genetic (16S rRNA, 16S-23S ITS, cpcB-IGS-cpcA, rpoC1, gyrB, rbcL, nifD loci) analyses. The description of Phormidium cf. irriguum CCALA 759 as the epitype of Phormidium irriguum was also used to complete the characterization of strain ETS-02.
蓝细菌（蓝藻门/蓝细菌纲，蓝藻纲）是分布最广泛的生物之一，能够适应不同的极端环境。这些微生物具有重要的生态作用，因为它们能够进行产氧光合作用，并且基于其产生多种生物活性化合物的能力而被应用于不同领域。它们的原核性质、许多隐存物种的存在以及不同命名系统的共存使得蓝细菌的分类鉴定特别困难。此外，对于几种物种来说，原始参考菌株（模式标本）缺失。越来越多的作者正在使用多相方法来表征蓝细菌，而典型化是最近用于解决其他微生物中模式标本缺失问题的一种趋势。在这里，我们重点研究一种丝状蓝细菌，它从尤加尼安热区（意大利帕多瓦）分离出来，暂命名为ETS - 02菌株，采用了一种多相方法，包括形态学、超微结构、生化（色素和脂肪酸含量）、生理学（固氮）和遗传学（16S rRNA、16S - 23S ITS、cpcB - IGS - cpcA、rpoC1、gyrB、rbcL、nifD基因座）分析。将近似灌溉席藻CCALA 759描述为灌溉席藻的后选模式标本也用于完成ETS - 02菌株的表征。
