Józsa R, Vigh S, Mess B, Schally A V
Cell Tissue Res. 1986;244(3):681-5. doi: 10.1007/BF00212549.
In chicken embryos of different ages and in young chickens after hatching, neural elements reacting with antibodies generated against synthetic ovine corticotropin-releasing factor (CRF) were studied by means of the peroxidase-anti-peroxidase (PAP) technique at the light-microscopic level. CRF-immunoreactivity was first observed in perikarya located in the periventricular part of the hypothalamus on the 14th day of the incubation period. CRF-containing neural elements were detected on the same day of incubation in the external zone of the median eminence, but not in all investigated animals. In extrahypothalamic sites, immunoreactive perikarya were demonstrable in the central gray of the mesencephalon on the 15th day of incubation. Furthermore, immunoreactive cells appeared in other brain regions such as nucleus accumbens and dorsomedial nucleus of the thalamus after hatching. The present observations provide information regarding the functional development of the hypothalamo-hypophyseal-adrenal axis in the chick embryo.
利用过氧化物酶抗过氧化物酶（PAP）技术，在光学显微镜水平上研究了不同日龄鸡胚及出壳后幼鸡中与针对合成羊促肾上腺皮质激素释放因子（CRF）产生的抗体发生反应的神经成分。在孵化期第14天，首次在下丘脑室周部分的神经细胞体中观察到CRF免疫反应性。在孵化的同一天，在正中隆起的外侧区检测到含CRF的神经成分，但并非在所有研究的动物中都能检测到。在孵化第15天，在中脑中央灰质的下丘脑外部位可证实有免疫反应性神经细胞体。此外，出壳后，在其他脑区如伏隔核和丘脑背内侧核中出现了免疫反应性细胞。本观察结果提供了有关鸡胚下丘脑-垂体-肾上腺轴功能发育的信息。
