Hahn Monique Cabral, Werlang Isabel Cristina Ribas, Rechenmacher Ciliana, Morais Rahuany Velleda de, Barbé-Tuana Florencia María, Grun Lucas Kich, Guma Fátima Theresinha Costa Rodrigues, Silva Clécio Homrich da, Bernardi Juliana Rombaldi, Michalowski Mariana Bohns, Goldani Marcelo Zubaran
Hospital de Clínicas de Porto Alegre, Laboratório de Pediatria Translacional, Núcleo de Estudos em Saúde da Criança e do Adolescente (NESCA), Porto Alegre, RS, Brazil.
Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-Graduação em Saúde da Criança e do Adolescente, Porto Alegre, RS, Brazil.
Genet Mol Biol. 2021 Dec 3;44(4):e20200411. doi: 10.1590/1678-4685-GMB-2020-0411. eCollection 2021.
Different intrauterine exposures are associated with different metabolic profiles leading to growth and development characteristics in children and also relate to health and disease patterns in adult life. The objective of this work was to evaluate the impact of four different intrauterine environments on the telomere length of newborns. This is a longitudinal observational study using a convenience sample of 222 mothers and their term newborns (>37 weeks of gestational age) from hospitals in Porto Alegre, Rio Grande do Sul (Brazil), from September 2011 to January 2016. Sample was divided into four groups: pregnant women with Gestational Diabetes Mellitus (DM) (n=38), smoking pregnant women (TOBACCO) (n=52), mothers with small-for-gestational age (SGA) children due to idiopathic intrauterine growth restriction (n=33), and a control group (n=99). Maternal and newborn genomic DNA were obtained from epithelial mucosal cells. Telomere length was assessed by qPCR, with the calculation of the telomere and single copy gene (T/S ratio). In this sample, there was no significant difference in telomere length between groups (p>0.05). There was also no association between childbirth weight and telomere length in children (p>0.05). For term newborns different intrauterine environments seems not to influence telomere length at birth.
不同的宫内暴露与不同的代谢特征相关，这些特征会导致儿童的生长发育特点，并且还与成年后的健康和疾病模式有关。这项研究的目的是评估四种不同的宫内环境对新生儿端粒长度的影响。这是一项纵向观察性研究，使用了2011年9月至2016年1月期间来自巴西南里奥格兰德州阿雷格里港医院的222名母亲及其足月儿（胎龄>37周）的便利样本。样本分为四组：患有妊娠期糖尿病（DM）的孕妇（n = 38）、吸烟孕妇（TOBACCO）（n = 52）、因特发性宫内生长受限而产下小于胎龄儿（SGA）的母亲（n = 33）以及一个对照组（n = 99）。从上皮粘膜细胞中获取母亲和新生儿的基因组DNA。通过qPCR评估端粒长度，并计算端粒与单拷贝基因的比值（T/S比值）。在这个样本中，各组之间的端粒长度没有显著差异（p>0.05）。儿童的出生体重与端粒长度之间也没有关联（p>0.05）。对于足月儿来说，不同的宫内环境似乎不会影响出生时的端粒长度。
