Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea.
Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea.
J Microbiol. 2023 Jun;61(6):603-613. doi: 10.1007/s12275-023-00051-7. Epub 2023 May 5.
Three novel bacterial strains, 321, 335, and 353, were isolated from the intestines of Aegosoma sinicum larvae collected from Paju-Si, South Korea. The strains were Gram-negative, obligate aerobe and had rod-shaped cells with a single flagellum. The three strains belonged to the genus Luteibacter in the family Rhodanobacteraceae and shared < 99.2% similarity in their 16S rRNA gene sequence and < 83.56% similarity in thier whole genome sequence. Strains 321, 335, and 353 formed a monophyletic clade with Luteibacter yeojuensis KACC 11405, L. anthropi KACC 17855, and L. rhizovicinus KACC 12830, with sequence similarities of 98.77-98.91%, 98.44-98.58%, and 97.88-98.02%, respectively. Further genomic analyses, including the construction of the Up-to-date Bacterial Core Gene (UBCG) tree and assessment of other genome-related indices, indicated that these strains were novel species belonging to the genus Luteibacter. All three strains contained ubiquinone Q8 as their major isoprenoid quinone and iso-C and summed feature 9 (C 10-methyl and/or iso-C ω9c) as their major cellular fatty acids. Phosphatidylethanolamine and diphosphatidylglycerol were the major polar lipids in all the strains. The genomic DNA G + C contents of strains 321, 335, and 353 were 66.0, 64.5, and 64.5 mol%, respectively. Based on multiphasic classification, strains 321, 335, and 353 were classified into the genus Luteibacter as the type strains of novel species, for which the names Luteibacter aegosomatis sp. nov., Luteibacter aegosomaticola sp. nov., and Luteibacter aegosomatissinici sp. nov. are proposed, respectively.
从韩国坡州市采集的中华大刀螳幼虫肠道中分离到 3 株新的细菌菌株，分别为 321、335 和 353。这些菌株均为革兰氏阴性、需氧杆状菌，具有单根鞭毛。这 3 株菌均属于罗单胞菌科鲁丹杆菌属，其 16S rRNA 基因序列相似性均小于 99.2%，全基因组序列相似性均小于 83.56%。菌株 321、335 和 353 与黄杆菌属的耶尔森氏黄杆菌 KACC 11405、人类黄杆菌 KACC 17855 和根瘤黄杆菌 KACC 12830 形成单系群，序列相似性分别为 98.77-98.91%、98.44-98.58%和 97.88-98.02%。进一步的基因组分析，包括构建最新的细菌核心基因 (UBCG) 树和评估其他与基因组相关的指标，表明这些菌株是属于黄杆菌属的新物种。这 3 株菌均含有泛醌 Q8 作为主要的异戊二烯醌，iso-C 和总和特征 9（C 10-甲基和/或 iso-C ω9c）作为主要的细胞脂肪酸。所有菌株均含有磷脂酰乙醇胺和双磷脂酰甘油作为主要的极性脂。菌株 321、335 和 353 的基因组 DNA G+C 含量分别为 66.0、64.5 和 64.5 mol%。基于多相分类，菌株 321、335 和 353 被分类为黄杆菌属，是新种的模式株，分别命名为中华大刀螳黄杆菌新种、中华大刀螳黄杆菌新亚种和中华大刀螳黄杆菌新种。
