Biochemical Research Center, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Biochemical Research Center, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Colloids Surf B Biointerfaces. 2022 Feb;210:112256. doi: 10.1016/j.colsurfb.2021.112256. Epub 2021 Dec 1.
In this study, ultrafine magnetic nanofibers were developed for bone regeneration purposes. Nanofibers were acquired by electrospinning using a two-component nanofiber matrix (CP: chitosan (Cs) and polyvinyl alcohol (PVA)) containing different concentrations of succinate conjugated-magnetic hydroxyapatite nanocomposites (SMHA). Hybrid nanofibers (CP&SMHA) containing 5 mg ml of SMHA nanocomposite showed well-defined properties in terms of physicochemical properties and cell behavior. Then, they were modified with adenosine 5'-triphosphate (ATP) and Mg ions. The initial adhesion of mesenchymal stem cells and their proliferation rate on the surface of modified nanofibers (Mg.ATP.CP&SMHA) were significantly increased as compared to those of bare nanofibers. Analysis of common osteogenic markers such as alkaline phosphatase activity and the expression of Runt-related transcription factor 2 and osteocalcin confirmed the osteogenic efficacy enhancement of CP&SMHA nanofibers when they were functionalized with ATP and Mg. The utilization of the antagonist of purine receptor, P2X7, revealed that this receptor has a major role in the osteogenesis process induced by Mg.ATP.CP&SMHA. Moreover, the results showed that cell adhesion, proliferation, and differentiation improved as nanofibers were under the influence of the electromagnetic field (EMF), displaying synergistic effects in the process of bone formation. Mg.ATP.CP&SMHA also showed an antibacterial effect against gram-negative and gram-positive bacteria, Escherichia coli and Staphylococcus aureus, respectively. Considering the high osteogenic potential and antibacterial activity of Mg.ATP.CP&SMHA nanofibers particularly in combination with EMF, it can serve as a great candidate for use in bone tissue engineering applications.
在这项研究中，开发了超精细磁性纳米纤维用于骨再生目的。通过使用含有不同浓度琥珀酸共轭磁性羟基磷灰石纳米复合材料（SMHA）的两亲性纳米纤维基质（CP：壳聚糖（Cs）和聚乙烯醇（PVA））的静电纺丝获得纳米纤维。含有 5mg/ml SMHA 纳米复合材料的杂化纳米纤维（CP＆SMHA）在物理化学性质和细胞行为方面表现出良好的特性。然后，它们用三磷酸腺苷（ATP）和镁离子进行修饰。与裸纳米纤维相比，间充质干细胞在修饰纳米纤维（Mg.ATP.CP＆SMHA）表面的初始粘附及其增殖率显著增加。分析碱性磷酸酶活性和 runt 相关转录因子 2 和骨钙素的表达等常见成骨标志物证实，当 CP＆SMHA 纳米纤维与 ATP 和 Mg 功能化时，增强了其成骨功效。嘌呤受体拮抗剂 P2X7 的利用表明该受体在 Mg.ATP.CP＆SMHA 诱导的成骨过程中起主要作用。此外，结果表明，随着纳米纤维受到电磁场（EMF）的影响，细胞粘附、增殖和分化得到改善，在骨形成过程中表现出协同作用。Mg.ATP.CP＆SMHA 对革兰氏阴性和革兰氏阳性菌，大肠杆菌和金黄色葡萄球菌分别具有抗菌作用。考虑到 Mg.ATP.CP＆SMHA 纳米纤维的高成骨潜力和抗菌活性，特别是与 EMF 结合使用时，它可以作为骨组织工程应用的良好候选物。
