Huang Danwei, Fitzhugh Kirk, Rouse Greg W
Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Cladistics. 2011 Aug;27(4):356-379. doi: 10.1111/j.1096-0031.2010.00343.x. Epub 2010 Dec 3.
Cladistic relationships among fabriciids have to date been explored in the context of adult morphology, but resolution has been declining as more species are described. In this study, we incorporated data on the reproductive system, including features related to the male sperm and sperm storage by females, to supplement existing data on adult morphology (for a total of 50 characters). Three nuclear DNA markers (18S rDNA approximately 1800 bp, the D1 region of 28S rDNA approximately 320 bp, and histone H3 approximately 330 bp) were sequenced from 21 species of fabriciids. We assessed the phylogeny of Fabriciidae based on an integrative analysis of these morphological and molecular characters. Our results show that, in addition to three previously recovered apomorphies for Fabriciidae (absence of ventral lips, modification of abdominal uncini to an elongate manubrium, and presence of branchial hearts), six more apomorphies associated with the reproductive system can be used to support this clade-spermiogenesis only in the thorax, spermiogenesis in large clusters with a central cytophore, single dorsal sperm duct, sperm nuclear projection, thickening of the sperm nuclear membrane and the sperm extra-axonemal sheath. The results require the erection of two new genera and two new species, which are described. © The Willi Hennig Society 2010.
迄今为止，叶形虫科内部的支序关系是在成虫形态学的背景下进行研究的，但随着更多物种被描述，分辨率一直在下降。在本研究中，我们纳入了有关生殖系统的数据，包括与雄性精子以及雌性精子储存相关的特征，以补充现有的成虫形态学数据（总共50个特征）。对21种叶形虫科物种的三个核DNA标记（约1800 bp的18S rDNA、约320 bp的28S rDNA的D1区域和约330 bp的组蛋白H3）进行了测序。我们基于对这些形态学和分子特征的综合分析评估了叶形虫科的系统发育。我们的结果表明，除了之前确定的叶形虫科的三个共衍征（腹唇缺失、腹部小钩演变为细长的柄以及鳃心的存在）之外，还有六个与生殖系统相关的共衍征可用于支持这个分支——仅在胸部发生精子形成、在具有中央胞质球的大簇中发生精子形成、单一的背侧精子管、精子核突出、精子核膜增厚以及精子轴外鞘增厚。结果需要建立两个新属和两个新物种，并对其进行描述。©威利·亨尼希学会2010年。
