• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状DENND4C通过上调BRD4信号通路促进肺癌的增殖和转移。

circDENND4C Promotes Proliferation and Metastasis of Lung Cancer by Upregulating BRD4 Signaling Pathway.

作者信息

Ma Dongjie, Qin Yingzhi, Li Shanqing, Li Li, He Jia, Chen Yeye, Zhou Xiaoyun, Liu Hongsheng

机构信息

Department of Thoracic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.

出版信息

J Oncol. 2021 Nov 28;2021:2469691. doi: 10.1155/2021/2469691. eCollection 2021.

DOI:10.1155/2021/2469691
PMID:34876902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8645384/
Abstract

OBJECTIVE

To investigate the effects of circDENND4C on the malignant biological behavior of lung cancer and its downstream target genes and molecular mechanisms.

METHODS

The expression of circDENND4C in lung cancer tissues and cells was detected. After transfection with silenced circDENND4C, the expression levels of circDENND4C, miR-141-3p, and BRD4 in lung cancer cells were detected by qRT-PCR. The targeting relationship between circDENND4C and miR-141-3p as well as miR-141-3p and BRD4 was verified. Cell activity was detected by CCK-8 and EdU assay. Transwell assay was used to detect the invasiveness of A549 and NCI-H1299 in each group. Effects of circDENND4C on proliferation and metastasis of lung cancer in nude mice were studied.

RESULTS

In vitro and in vivo results showed that circDENND4C silencing reduced the proliferation, invasion, and metastasis of lung cancer cells. Mechanism studies showed that circDENND4C has a targeting relationship with miR-141-3p. However, miR-141-3p has a targeting relationship with BRD4. circDENND4C indirectly upregulated BRD4 through sponge adsorption of miR-141-3p, thereby promoting metastasis and proliferation of NSCLC.

CONCLUSION

circDENND4C, as an oncogene, promotes the proliferation, invasion, and metastasis of lung cancer cells.

摘要

目的

探讨环状DENND4C（circDENND4C）对肺癌恶性生物学行为及其下游靶基因和分子机制的影响。

方法

检测circDENND4C在肺癌组织和细胞中的表达。转染沉默circDENND4C后，采用qRT-PCR检测肺癌细胞中circDENND4C、miR-141-3p和BRD4的表达水平。验证circDENND4C与miR-141-3p以及miR-141-3p与BRD4之间的靶向关系。通过CCK-8和EdU检测法检测细胞活性。采用Transwell检测法检测各组A549和NCI-H1299细胞的侵袭能力。研究circDENND4C对裸鼠肺癌增殖和转移的影响。

结果

体外和体内实验结果表明，沉默circDENND4C可降低肺癌细胞的增殖、侵袭和转移能力。机制研究表明，circDENND4C与miR-141-3p存在靶向关系。然而，miR-141-3p与BRD4存在靶向关系。circDENND4C通过海绵吸附miR-141-3p间接上调BRD4，从而促进非小细胞肺癌的转移和增殖。

结论

circDENND4C作为一种癌基因，可促进肺癌细胞的增殖、侵袭和转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/7c0cf6d4b660/JO2021-2469691.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/a98813925e43/JO2021-2469691.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/d5b2b376d239/JO2021-2469691.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/c6b3402eb0e2/JO2021-2469691.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/9a196185ea0e/JO2021-2469691.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/2b7f19764910/JO2021-2469691.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/7c0cf6d4b660/JO2021-2469691.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/a98813925e43/JO2021-2469691.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/d5b2b376d239/JO2021-2469691.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/c6b3402eb0e2/JO2021-2469691.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/9a196185ea0e/JO2021-2469691.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/2b7f19764910/JO2021-2469691.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/266c/8645384/7c0cf6d4b660/JO2021-2469691.006.jpg

相似文献

1
circDENND4C Promotes Proliferation and Metastasis of Lung Cancer by Upregulating BRD4 Signaling Pathway.环状DENND4C通过上调BRD4信号通路促进肺癌的增殖和转移。
J Oncol. 2021 Nov 28;2021:2469691. doi: 10.1155/2021/2469691. eCollection 2021.
2
Circular RNA circ_0074027 indicates a poor prognosis for NSCLC patients and modulates cell proliferation, apoptosis, and invasion via miR-185-3p mediated BRD4/MADD activation.环状 RNA circ_0074027 提示 NSCLC 患者预后不良，并通过 miR-185-3p 介导的 BRD4/MADD 激活调节细胞增殖、凋亡和侵袭。
J Cell Biochem. 2020 Mar;121(3):2632-2642. doi: 10.1002/jcb.29484. Epub 2019 Nov 3.
3
Circular RNA circDENND4C facilitates proliferation, migration and glycolysis of colorectal cancer cells through miR-760/GLUT1 axis.环状 RNA circDENND4C 通过 miR-760/GLUT1 轴促进结直肠癌细胞的增殖、迁移和糖酵解。
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2387-2400. doi: 10.26355/eurrev_202003_20506.
4
MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway.微小RNA-330-3p通过激活丝裂原活化蛋白激酶/细胞外信号调节激酶（MAPK/ERK）信号通路，作用于谷氨酸受体离子otropic AMPA 3型（GRIA3），从而促进非小细胞肺癌的细胞侵袭和转移。
J Hematol Oncol. 2017 Jun 19;10(1):125. doi: 10.1186/s13045-017-0493-0.
5
Circ_0000376 downregulation inhibits the progression of non-small cell lung cancer by mediating the miR-488-3p/BRD4 axis and the PI3K/PKB signaling pathway.Circ_0000376下调通过介导miR-488-3p/BRD4轴和PI3K/PKB信号通路抑制非小细胞肺癌进展。
Histol Histopathol. 2021 Dec;36(12):1309-1324. doi: 10.14670/HH-18-390. Epub 2021 Nov 3.
6
LncRNA NORAD, sponging miR-363-3p, promotes invasion and EMT by upregulating PEAK1 and activating the ERK signaling pathway in NSCLC cells.长链非编码 RNA NORAD 通过海绵吸附 miR-363-3p，上调 PEAK1 并激活 ERK 信号通路，促进非小细胞肺癌细胞的侵袭和 EMT。
J Bioenerg Biomembr. 2021 Jun;53(3):321-332. doi: 10.1007/s10863-021-09892-6. Epub 2021 Mar 19.
7
Knockdown of circDENND4C inhibits glycolysis, migration and invasion by up-regulating miR-200b/c in breast cancer under hypoxia.敲低 circDENND4C 通过上调低氧下乳腺癌中的 miR-200b/c 抑制糖酵解、迁移和侵袭。
J Exp Clin Cancer Res. 2019 Sep 5;38(1):388. doi: 10.1186/s13046-019-1398-2.
8
circ_0007385 served as competing endogenous RNA for miR-519d-3p to suppress malignant behaviors and cisplatin resistance of non-small cell lung cancer cells.circ_0007385 作为竞争性内源性 RNA，与 miR-519d-3p 结合，抑制非小细胞肺癌细胞的恶性行为和顺铂耐药性。
Thorac Cancer. 2020 Aug;11(8):2196-2208. doi: 10.1111/1759-7714.13527. Epub 2020 Jun 29.
9
Circ_100565 promotes proliferation, migration and invasion in non-small cell lung cancer through upregulating HMGA2 via sponging miR-506-3p.Circ_100565通过海绵化miR-506-3p上调HMGA2，从而促进非小细胞肺癌的增殖、迁移和侵袭。
Cancer Cell Int. 2020 May 12;20:160. doi: 10.1186/s12935-020-01241-8. eCollection 2020.
10
MicroRNA-338-3p suppresses cell proliferation and induces apoptosis of non-small-cell lung cancer by targeting sphingosine kinase 2.微小RNA-338-3p通过靶向鞘氨醇激酶2抑制非小细胞肺癌细胞增殖并诱导其凋亡。
Cancer Cell Int. 2017 Apr 17;17:46. doi: 10.1186/s12935-017-0415-9. eCollection 2017.

引用本文的文献

1
circDENND4C serves as a sponge for miR-200b to drive non-small cell lung cancer advancement by regulating MMP-9 expression.环状DENND4C作为miR-200b的海绵，通过调节MMP-9表达来驱动非小细胞肺癌进展。
Front Oncol. 2025 Feb 17;15:1441384. doi: 10.3389/fonc.2025.1441384. eCollection 2025.
2
Circular RNA in Non-small Cell Lung Carcinoma: Identification of Targets and New Treatment Modalities.环状 RNA 在非小细胞肺癌中的作用：靶标鉴定和新的治疗方式。
Cancer Genomics Proteomics. 2023 Dec;20(6suppl):646-668. doi: 10.21873/cgp.20413.
3
Context-Dependent Regulation of Gene Expression by Non-Canonical Small RNAs.

本文引用的文献

1
Cancer Statistics, 2021.癌症统计数据，2021.
CA Cancer J Clin. 2021 Jan;71(1):7-33. doi: 10.3322/caac.21654. Epub 2021 Jan 12.
2
The Effect of Advances in Lung-Cancer Treatment on Population Mortality.肺癌治疗进展对人群死亡率的影响。
N Engl J Med. 2020 Aug 13;383(7):640-649. doi: 10.1056/NEJMoa1916623.
3
Cartilage Oligomeric Matrix Protein promotes epithelial-mesenchymal transition by interacting with Transgelin in Colorectal Cancer.软骨寡聚基质蛋白通过与结直肠癌细胞中的 Transgelin 相互作用促进上皮-间充质转化。
非经典小RNA对基因表达的上下文依赖性调控
Noncoding RNA. 2022 Apr 29;8(3):29. doi: 10.3390/ncrna8030029.
Theranostics. 2020 Jul 9;10(19):8790-8806. doi: 10.7150/thno.44456. eCollection 2020.
4
Circular RNA circDENND4C facilitates proliferation, migration and glycolysis of colorectal cancer cells through miR-760/GLUT1 axis.环状 RNA circDENND4C 通过 miR-760/GLUT1 轴促进结直肠癌细胞的增殖、迁移和糖酵解。
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2387-2400. doi: 10.26355/eurrev_202003_20506.
5
Overexpression of circular RNA hsa_circ_0001038 promotes cervical cancer cell progression by acting as a ceRNA for miR-337-3p to regulate cyclin-M3 and metastasis-associated in colon cancer 1 expression.环状 RNA hsa_circ_0001038 的过表达通过作为 miR-337-3p 的 ceRNA 来调节 cyclin-M3 和结肠癌转移相关蛋白 1 的表达，促进宫颈癌细胞的进展。
Gene. 2020 Apr 5;733:144273. doi: 10.1016/j.gene.2019.144273. Epub 2019 Dec 3.
6
ACT001, a novel PAI-1 inhibitor, exerts synergistic effects in combination with cisplatin by inhibiting PI3K/AKT pathway in glioma.ACT001，一种新型的 PAI-1 抑制剂，通过抑制脑胶质瘤中的 PI3K/AKT 通路，与顺铂联合发挥协同作用。
Cell Death Dis. 2019 Oct 7;10(10):757. doi: 10.1038/s41419-019-1986-2.
7
Knockdown of circDENND4C inhibits glycolysis, migration and invasion by up-regulating miR-200b/c in breast cancer under hypoxia.敲低 circDENND4C 通过上调低氧下乳腺癌中的 miR-200b/c 抑制糖酵解、迁移和侵袭。
J Exp Clin Cancer Res. 2019 Sep 5;38(1):388. doi: 10.1186/s13046-019-1398-2.
8
Past, present, and future of circRNAs.环状 RNA 的过去、现在和未来。
EMBO J. 2019 Aug 15;38(16):e100836. doi: 10.15252/embj.2018100836. Epub 2019 Jul 25.
9
Joint bioinformatics analysis of underlying potential functions of hsa-let-7b-5p and core genes in human glioma.人类脑胶质瘤中 hsa-let-7b-5p 和核心基因的潜在功能的联合生物信息学分析。
J Transl Med. 2019 Apr 17;17(1):129. doi: 10.1186/s12967-019-1882-7.
10
The novel roles of circRNAs in human cancer.环状 RNA 在人类癌症中的新作用。
Mol Cancer. 2019 Jan 9;18(1):6. doi: 10.1186/s12943-018-0934-6.