Zhang Guoli, Han Yan, Wang Huan, Wang Ziyang, Xiao Hongxing, Sun Mingzhou
Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
Qian'an No. 1 Middle School, Tangshan 063000, China.
AoB Plants. 2021 Nov 1;13(6):plab070. doi: 10.1093/aobpla/plab070. eCollection 2021 Dec.
Quaternary climate oscillations and complex topography have tremendous effects on current distribution and genetic structure of species, and hence the Qinghai-Tibet Plateau (QTP), the largest plateau in the world, has become a hotspot for many phylogeographic studies. However, little is known about the phylogeographic pattern of herbaceous plants in QTP. Here, we investigate the genetic diversity, population structure and historical dynamics of , using five chloroplast DNA (cpDNA) fragments and seven microsatellite markers. A total of 15 populations, and 149 individuals were sampled throughout the QTP. High genetic diversity was detected both in cpDNA ( = 0.820) and SSR ( = 0.689, = 0.699). Ten cpDNA haplotypes and 163 alleles were identified. AMOVA and clustering analyses revealed obvious differentiation between regions. The , and Mantel test showed significant phylogeographic structure of . The neutrality test and mismatch distribution analyses indicated that could not have undergone a historical population expansion, but population XS from the Qilian Mountain area could have experienced a local expansion. Bottleneck analyses indicated that had not experienced bottleneck recently. Based on cpDNA and SSR results, the Qilian Mountain area was inferred as a potential glacial refuge, and the southern Tibet valley was considered as a 'microrefugia' for . These findings provided new insights into the location of glacial refuges for the species distributed in QTP, and supplemented more plant species data for the response of QTP species to the Quaternary climate.
第四纪气候振荡和复杂地形对物种的当前分布和遗传结构产生了巨大影响，因此，世界上最大的高原——青藏高原已成为许多系统地理学研究的热点。然而，关于青藏高原草本植物的系统地理格局却知之甚少。在此，我们利用五个叶绿体DNA（cpDNA）片段和七个微卫星标记，研究了[物种名称未给出]的遗传多样性、种群结构和历史动态。在青藏高原共采集了15个种群的149个个体。在cpDNA（ = 0.820）和SSR（ = 0.689， = 0.699）中均检测到高遗传多样性。鉴定出10个cpDNA单倍型和163个等位基因。方差分析（AMOVA）和聚类分析揭示了不同区域之间存在明显分化。[相关统计量及检验未明确写出具体内容]表明[物种名称未给出]具有显著的系统地理结构。中性检验和失配分布分析表明，[物种名称未给出]不可能经历过历史种群扩张，但祁连山区的XS种群可能经历过局部扩张。瓶颈分析表明，[物种名称未给出]最近没有经历过瓶颈。基于cpDNA和SSR结果，推断祁连山区是一个潜在的冰川避难所，藏南谷地被认为是[物种名称未给出]的一个“微避难所”。这些发现为分布在青藏高原的物种的冰川避难所位置提供了新的见解，并补充了更多关于青藏高原物种对第四纪气候响应的植物物种数据。
