Zhu Yulian, Yuen Michael, Li Wenxia, Yuen Hywel, Wang Min, Smith Deandrae, Peng Qiang
College of Food Science and Engineering, Northwest A & F University, Yangling, 712100, China.
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, 100048, China.
Curr Res Food Sci. 2021 Nov 25;4:840-851. doi: 10.1016/j.crfs.2021.11.008. eCollection 2021.
Procyanidin is an important polyphenol for its health-promoting properties, however, the study of procyanidin in sea buckthorn was limited. In this paper, sea buckthorn procyanidin (SBP) was obtained through a green isolation and enrichment technique with an extraction rate and purity of 9.1% and 91.5%. The structure of SBP was analyzed using Ultraviolet-visible spectroscopy (UV-vis), Fourier-transform infrared spectroscopy (FT-IR), and liquid chromatography-mass spectrometry (LC-MS/MS). The results show that SBP is an oligomeric procyanidin, mainly composed of (-)-epicatechin gallate, procyanidin B, (+)-gallocatechin-(+)-catechin, and (+)-gallocatechin dimer. SBP showed superior scavenging capacity on free radicals. Furthermore, the cleaning rate of the ABTS radical was 4.8 times higher than vitamin C at the same concentration. Moreover, SBP combined with vitamin C presented potent synergistic antioxidants with combined index values below 0.3 with concentration rates from 5:5 to 2:8. SBP also provided significant protection against oxidative stress caused by hydrogen peroxide (HO) on RAW264.7 cells. These findings prove the potential of SBP as a natural antioxidant in food additives and support the in-depth development of sea buckthorn resources.
原花青素因其促进健康的特性而成为一种重要的多酚类物质，然而，沙棘中原花青素的研究却较为有限。本文通过绿色分离富集技术获得了沙棘原花青素（SBP），其提取率和纯度分别为9.1%和91.5%。利用紫外可见光谱（UV-vis）、傅里叶变换红外光谱（FT-IR）和液相色谱-质谱联用（LC-MS/MS）对SBP的结构进行了分析。结果表明，SBP是一种低聚原花青素，主要由（-）-表儿茶素没食子酸酯、原花青素B、（+）-儿茶素-（+）-表儿茶素和（+）-儿茶素二聚体组成。SBP对自由基表现出优异的清除能力。此外，在相同浓度下，SBP对ABTS自由基的清除率比维生素C高4.8倍。而且，SBP与维生素C组合呈现出强大的协同抗氧化剂作用，在浓度比为5:5至2:8时，组合指数值低于0.3。SBP还对过氧化氢（HO）诱导的RAW264.7细胞氧化应激提供了显著保护。这些发现证明了SBP作为食品添加剂中天然抗氧化剂的潜力，并支持了沙棘资源的深入开发。
