Dienaitė Lijana, Pukalskas Audrius, Pukalskienė Milda, Pereira Carolina V, Matias Ana A, Venskutonis Petras Rimantas
Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų˛ pl. 19, LT-50254 Kaunas, Lithuania.
IBET-Instituto de Biologia Experimental e Tecnológica, Food & Health Division Apartado 12, 2780-901 Oeiras, Portugal.
Antioxidants (Basel). 2020 Mar 25;9(4):274. doi: 10.3390/antiox9040274.
This study aimed at valorisation of sea buckthorn pomace (SBP) for the production of extracts containing valuable bioactive compounds. For this purpose, SBP defatted by supercritical CO was subjected to consecutive fractionation with pressurized ethanol and water, which yielded 11.9% and 4.8% of extracts, respectively. The extracts were evaluated for their antioxidant potential, phytochemical composition and antiproliferative effects against cancer cells. Water extracts exhibited remarkably higher values in Folin-Ciocalteu assay of total phenolic content, oxygen radical absorbance capacity (ORAC), ABTS/DPPH scavenging and cellular antioxidant activity (CAA) assays and more efficiently inhibited proliferation of HT29 cells at non-cytotoxic concentrations measured in non-tumoral Caco2 cells. Among 28 detected and 21 quantified phytochemicals, flavonols with the structures of isorhamnetin (five compounds), quercetin (three compounds), kaempferol (three compounds) glycosides and catechin (six compounds) were the most abundant in the extracts. In conclusion, the applied method of fractionation of SBP produces promising natural antioxidant complexes with antiproliferative properties that could find potential applications in nutraceuticals, functional foods and cosmeceuticals.
本研究旨在对沙棘果渣进行增值利用，以生产含有珍贵生物活性化合物的提取物。为此，用超临界二氧化碳脱脂的沙棘果渣先后用加压乙醇和水进行分级分离，分别得到了11.9%和4.8%的提取物。对提取物的抗氧化潜力、植物化学成分以及对癌细胞的抗增殖作用进行了评估。在总酚含量的福林-酚法、氧自由基吸收能力（ORAC）、ABTS/DPPH清除率和细胞抗氧化活性（CAA）测定中，水提取物的值显著更高，并且在非肿瘤性Caco2细胞中测得的非细胞毒性浓度下，能更有效地抑制HT29细胞的增殖。在检测到的28种和定量的21种植物化学成分中，提取物中含量最丰富的是具有异鼠李素（5种化合物）、槲皮素（3种化合物）、山奈酚（3种化合物）糖苷和儿茶素（6种化合物）结构的黄酮醇。总之，所应用的沙棘果渣分级分离方法产生了具有抗增殖特性的有前景的天然抗氧化复合物，这些复合物可能在营养保健品、功能性食品和药妆品中找到潜在应用。
