Precision Medicine Center, Beilun People's Hospital, Zhejiang University School of Medicine First Affiliated Hospital Beilun Branch, Ningbo, Zhejiang, 315806, P. R. China.
Department of Clinical Laboratory, Zhejiang University School of Medicine First Affiliated Hospital, Hangzhou, Zhejiang, 310003, P. R. China.
Anal Methods. 2021 Dec 24;14(1):7-16. doi: 10.1039/d1ay01570b.
The detection of biomolecular analytes is of great importance in clinical, environmental, and argo-food areas, among which the electrochemical methodology is attracting much attention. In particular, screen-printed electrode (SPE)-based sensing applications have exhibited potential possibility for on-site detection, especially for fast clinical biomarker detection, since they provide a miniaturized but robust and portable electrode detection system. In this context, we focused on the modification of SPE with functional antibodies to improve the electrochemical detection performance in versatile sensing applications, particularly for COVID-19 detection. These antibodies were immobilized onto the electrode surface various methodologies, through which the powerful potential from the modification of SPE was revealed. Finally, more novel and excellent works on the biomolecular modification of SPE and the prospects of this technology from its state-of-art status to commercialization are previewed and future perspectives in this field are mentioned.
生物分子分析物的检测在临床、环境和农业食品领域都非常重要，其中电化学方法受到了广泛关注。特别是，基于丝网印刷电极（SPE）的传感应用具有现场检测的潜在可能性，特别是对于快速临床生物标志物检测，因为它们提供了一种小型化但强大且便携的电极检测系统。在这种情况下，我们专注于通过功能抗体修饰 SPE，以提高在各种传感应用中的电化学检测性能，特别是用于 COVID-19 检测。这些抗体通过各种方法固定在电极表面上，从而揭示了 SPE 修饰的强大潜力。最后，对 SPE 的生物分子修饰的更多新颖和出色的工作，以及从其最先进状态到商业化的这项技术的前景进行了展望，并提到了该领域的未来展望。
