球形赖氨酸芽孢杆菌（Sporosarcina beigongshangi），从白酒窖泥中分离得到。
Sporosarcina beigongshangi sp. nov., isolated from pit mud of Baijiu.
机构信息
Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing, 100048, China.
Operations and Management Department, Beijing Sanyuan Foods Co., Ltd, Beijing, 100076, China.
出版信息
Arch Microbiol. 2021 Dec 8;204(1):10. doi: 10.1007/s00203-021-02601-2.
A Gram-positive, aerobic and short rod-shaped bacterium designated REN13, was isolated from pit mud of Baijiu, in Sichuan province, China. Strain REN13 could grow at 10-50 ℃, pH 6.0-9.0 and 0-2% (w/v) NaCl, with the optimal growth occurred at 28 ℃, pH 7.0, and 2% (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain REN13 was closely related to Sporosarcina globispora DSM 4 (98.6%). The DNA G + C content of strain REN13 was 41.1 mol %. DDH and ANI value between strain REN13 and S. globispora DSM 4 was 24.4% and 67.6%, respectively. The major fatty acids were iso-C and antesio-C. The respiratory quinone was MK-7, and the polar lipids were phosphatidylethanolamine, phospholipids, phosphatidylglycerol and diphosphatidylglycerol. Based on the above polyphasic taxonomic analysis, strain REN13 represents a novel species of the genus Sporosarcina, for which the name Sporosarcina beigongshangi sp. nov. is proposed. The type strain is strain REN13 (= JCM 34409 = GDMCC 1.2151).
一株革兰氏阳性、需氧、短杆状细菌，命名为 REN13，从中国四川省白酒窖泥中分离得到。菌株 REN13 可以在 10-50℃、pH6.0-9.0 和 0-2%（w/v）NaCl 下生长，最适生长温度为 28℃、pH7.0 和 2%（w/v）NaCl。16S rRNA 基因序列分析表明，菌株 REN13 与球形芽孢杆菌 DSM 4（98.6%）密切相关。菌株 REN13 的 DNA G+C 含量为 41.1 mol%。REN13 株与 S. globispora DSM 4 之间的 DDH 和 ANI 值分别为 24.4%和 67.6%。主要脂肪酸为 iso-C 和 anteiso-C。呼吸醌为 MK-7，极性脂类为磷脂酰乙醇胺、磷脂、磷脂酰甘油和双磷脂酰甘油。基于以上多相分类学分析，菌株 REN13 代表球形芽孢杆菌属的一个新种，命名为 Sporosarcina beigongshangi sp. nov。其模式菌株为 REN13 株（=JCM 34409=GDMCC 1.2151）。
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