Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada.
Transfusion. 2022 Feb;62(2):448-456. doi: 10.1111/trf.16757. Epub 2021 Dec 8.
The biophysical properties of red blood cells (RBCs) provide potential biomarkers for the quality of donated blood. Blood unit segments provide a simple and nondestructive way to sample RBCs in clinical studies of transfusion efficacy, but it is not known whether RBCs sampled from segments accurately represent the biophysical properties of RBCs in blood bags.
RBCs were sampled from blood bags and segments every two weeks during 8 weeks of storage at 4°C. RBC deformability was measured by deformability-based sorting using the microfluidic ratchet device in order to derive a rigidity score. Standard hematological parameters, including mean corpuscular volume (MCV), red cell distribution width (RDW), mean cell hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and hemolysis were measured at the same time points.
Deformability of RBCs stored in blood bags was retained over 4 weeks storage, but a progressive loss of deformability was observed at weeks 6 and 8. This trend was mirrored in blood unit segments with a strong correlation to the blood bag data. Strong correlations were also observed between blood bag and segment for MCV, MCHC, and MCH but not for hemolysis.
RBCs sampled from blood unit segments accurately represent the biophysical properties of RBCs in blood bags but not hemolysis. Blood unit segments provide a simple and nondestructive sample for measuring RBC biophysical properties in clinical studies.
红细胞（RBC）的生物物理特性可为献血质量提供潜在的生物标志物。血液单元段为在输血疗效的临床研究中对 RBC 进行采样提供了一种简单且非破坏性的方法，但尚不清楚从段中采样的 RBC 是否准确代表了血袋中 RBC 的生物物理特性。
在 4°C 下储存 8 周的过程中，每两周从血袋和段中采集 RBC。使用微流控棘轮装置通过基于变形的分类来测量 RBC 的变形性，以便得出刚性评分。同时测量标准血液学参数，包括平均红细胞体积（MCV）、红细胞分布宽度（RDW）、平均细胞血红蛋白（MCH）、平均红细胞血红蛋白浓度（MCHC）和溶血。
在 4 周的储存过程中，保存在血袋中的 RBC 的变形性得以保留，但在第 6 和 8 周观察到变形性逐渐丧失。这一趋势与血液单元段中的趋势一致，与血袋数据具有很强的相关性。血袋和段之间也观察到 MCV、MCHC 和 MCH 之间的强相关性，但与溶血无关。
从血液单元段中采样的 RBC 准确地代表了血袋中 RBC 的生物物理特性，但不能代表溶血。血液单元段为在临床研究中测量 RBC 生物物理特性提供了一种简单且非破坏性的样本。
