Liu Qi, Wang Shichun, Yan Jie, Diao Ronghua, Huang Haishui, Xu Feng, Yao Chunyan
Department of Blood Transfusion, First Affiliated Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, P.R. China.
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, P.R. China.
J Biol Eng. 2025 May 6;19(1):40. doi: 10.1186/s13036-025-00510-2.
Reducing cell metabolism by lowering the storage temperature is an important method to improve the quality of stored RBCs and prolong the stored shelf life of RBCs. Traditional cryopreservation suffers from limitations such as tedious cytotoxic cryoprotectants (CPA) loading, unloading and ice-induced damage. Storage around 2-6 °C is an alternative method but only works for a short period due to significant storage lesions at this high storage temperature. We developed an improved supercooling preservation system for large-volume (100 ml) RBC suspensions in commercial polyvinylchloride (PVC) blood bags by minimizing favorable sites of ice nucleation and maintaining precise thermal control at -8 °C. This engineered protocol significantly reduces hemolysis, metabolic degradation, and oxidative stress while preserving RBC membrane integrity and functionality for up to 63 days. In vivo transfusion studies in New Zealand white rabbits demonstrate that supercooling-preserved RBCs achieve higher post-transfusion recovery rates, outperforming conventional storage methods. Our scalable and cost-effective supercooling system address critical needs for improving the quality of stored RBCs by achieving ice-free preservation, which representing a significant breakthrough in transfusion medicine.
通过降低储存温度来降低细胞代谢是提高储存红细胞质量和延长红细胞储存保质期的重要方法。传统的冷冻保存存在一些局限性，如繁琐的细胞毒性冷冻保护剂（CPA）加载、卸载以及冰诱导损伤。在2-6°C左右储存是一种替代方法，但由于在此高储存温度下会出现显著的储存损伤，所以只能在短时间内有效。我们通过最小化冰核形成的有利位点并在-8°C下保持精确的温度控制，开发了一种用于商业聚氯乙烯（PVC）血袋中大容量（100 ml）红细胞悬液的改进型过冷保存系统。这种设计方案显著降低了溶血、代谢降解和氧化应激，同时在长达63天的时间内保持红细胞膜的完整性和功能。在新西兰白兔身上进行的体内输血研究表明，过冷保存的红细胞在输血后实现了更高的回收率，优于传统的储存方法。我们可扩展且经济高效的过冷系统通过实现无冰保存满足了提高储存红细胞质量的关键需求，这代表了输血医学的一项重大突破。
