用于传感和医疗保健应用的 DNAzyme 的高级选择方法。
Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications.
机构信息
Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-Haryana, 125001, India; Department of Civil Engineering, College of Engineering, University of Nebraska at Lincoln, PO Box 886105, Lincoln, NE 68588-6105, USA.
Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-Haryana, 125001, India.
出版信息
Trends Biochem Sci. 2019 Mar;44(3):190-213. doi: 10.1016/j.tibs.2018.11.001. Epub 2018 Dec 14.
DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.
由于在传感和生物医学设备等众多应用中具有出色的催化活性，DNA 酶得到了广泛的研究。这些新发现的应用为设计新型催化 DNA 酶带来了很高的期望。然而，选择高效的 DNA 酶是一个具有挑战性但至关重要的过程。最初，指数富集的配体系统进化（SELEX）是一个劳动密集型和耗时的过程，但最近的进展加速了 DNA 酶分子的自动化生成。本综述总结了提高 DNA 酶亲和力和特异性的 SELEX 最新进展。新一代 DNA 酶的蓬勃发展有望为多种医疗保健应用打开大门。因此，本综述的很大一部分专门介绍了 DNA 酶在传感、治疗和纳米器件等各种生物学应用。此外，还进一步讨论了这些 DNA 酶在实际应用中遇到的障碍，为未来的研究提供了基础。
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