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不同剂量完全弗氏佐剂对模拟类风湿关节炎的多关节炎大鼠模型痛觉行为和炎症参数的影响。

Effects of different doses of complete Freund's adjuvant on nociceptive behaviour and inflammatory parameters in polyarthritic rat model mimicking rheumatoid arthritis.

机构信息

Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia Health Campus, Kelantan, Malaysia.

Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia.

出版信息

PLoS One. 2021 Dec 8;16(12):e0260423. doi: 10.1371/journal.pone.0260423. eCollection 2021.

DOI:10.1371/journal.pone.0260423
PMID:34879087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8654228/
Abstract

Complete Freund's adjuvant (CFA) has been used to develop the arthritic or inflammatory condition in the animal, but there is a lack of information concerning high CFA doses on nociceptive behaviour and inflammatory parameters. This study aimed to compare the effects of different high doses of CFA in rat to closely mimic nociceptive and inflammatory parameters of rheumatoid arthritis (RA) in humans. Twenty-four male Sprague-Dawley rats were randomly divided into four groups (n = 6): Control (C), CFA-induced polyarthritic groups at 5.0 mg/mL (CFA 5.0), 7.5 mg/mL (CFA 7.5) and 10.0mg/mL (CFA 10.0). The rats' right hindpaw was inoculated with CFA intradermally and developed into a polyarthritic state within 20 days. Nociceptive behavioural assessments, including von Frey and hot plate tests and spontaneous activities, were conducted on day 0, 7, 15 and 20. Bilateral ankle joints diameter and circumference, full blood count, joints and paw histological examinations were also conducted throughout the study period. Based on the results, CFA 5.0 and CFA 7.5 groups showed a significant increase in spontaneous activities and development of thermal hyperalgesia but no change in body weight and food intake, no development of tactile allodynia and haematological indices, and no significant morphological changes of joints histology. Meanwhile, CFA 10.0 group demonstrated significant and constant changes in all nociceptive and inflammatory parameters investigated. In conclusion, CFA at the dose of 10mg/mL has the most potential and reliable dosage to develop polyarthritis in a rat model to mimic RA condition in humans.

摘要

完全弗氏佐剂（CFA）已被用于在动物中产生关节炎或炎症状态，但关于高剂量 CFA 对伤害感受行为和炎症参数的影响的信息较少。本研究旨在比较不同高剂量 CFA 在大鼠中的作用，以模拟人类类风湿关节炎（RA）的伤害感受和炎症参数。24 只雄性 Sprague-Dawley 大鼠随机分为四组（n = 6）：对照组（C）、CFA 诱导的多关节炎组（CFA 5.0、CFA 7.5 和 CFA 10.0）。大鼠右后足底皮内接种 CFA，20 天内发展为多关节炎状态。在第 0、7、15 和 20 天进行伤害感受行为评估，包括 von Frey 和热板测试以及自发活动。还在整个研究期间进行双侧踝关节直径和周长、全血细胞计数、关节和爪子组织学检查。根据结果，CFA 5.0 和 CFA 7.5 组显示自发活动显著增加，热痛觉过敏发展，但体重和食物摄入无变化，触觉过敏和血液学指标无变化，关节组织学无明显形态变化。同时，CFA 10.0 组显示所有研究的伤害感受和炎症参数均发生显著且持续的变化。总之，CFA 的剂量为 10mg/mL 时最有可能发展为大鼠多关节炎模型，以模拟人类 RA 状况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7259/8654228/f8f5c9e1c733/pone.0260423.g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7259/8654228/f8f5c9e1c733/pone.0260423.g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7259/8654228/2ef35f7c7791/pone.0260423.g008.jpg
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