Naz Rabiya, Ahmed Zaheer, Shahzad Muhammad, Shabbir Arham, Kamal Faiza
Department of Home and Health Sciences, Allama Iqbal Open University Islamabad, Islamabad, Pakistan.
Department of Pharmacology, University of Health Sciences Lahore, Lahore, Pakistan.
Evid Based Complement Alternat Med. 2020 Nov 7;2020:8869484. doi: 10.1155/2020/8869484. eCollection 2020.
(cashew) has been used in the traditional system of medicine for curing many inflammatory disorders. The present study investigates the antiarthritic effects of cashew leaves extract using the rat model of FCA-induced rheumatoid arthritis. Arthritic rats were treated with 100 and 200 mg/kg b.w. ethanolic extract of cashew leaves. Animals were sacrificed at day 23, and before sacrificing the animals, gross pathological changes were observed. Histopathology of ankle joint was evaluated with hematoxylin and eosin staining, whereas the serum levels of C-reactive protein (CRP) were evaluated by the agglutination method. Inflammatory cells and other hematological parameters were assessed by employing an automated hemocytometer and chemistry analyzer. Rheumatoid factor (Rf) and lysosomal enzymes levels were determined in blood. Results indicated that significantly decreased the CPR levels, macroscopic arthritic score, and rheumatoid factor as compared to the diseased group. Histopathological evaluation showed significant attenuation in bone erosion, joint inflammation, and pannus formation by plant extract. Treatment with significantly suppressed the levels of acid phosphatase, -galactosidase, -glucuronidase, -acetylglucosaminidase, and collagenase. Moreover, significantly raised the HB levels and RBCs counts which were found depleted in the diseased group. The raised counts of total leukocytes, platelets, neutrophils, lymphocytes, and monocytes were also significantly decreased by treatment with plant extract. Comparative analysis showed that higher dose of demonstrated superior amelioration of rheumatoid arthritis as compared to low dose. In conclusion, possesses significant antiarthritic potential, which may be attributed to the suppression of lysosomal enzymes and collagenase levels.
（腰果）已被用于传统医学体系中治疗多种炎症性疾病。本研究使用弗氏完全佐剂诱导的类风湿性关节炎大鼠模型，研究腰果叶提取物的抗关节炎作用。用100和200mg/kg体重的腰果叶乙醇提取物治疗关节炎大鼠。在第23天处死动物，在处死动物前，观察大体病理变化。用苏木精和伊红染色评估踝关节的组织病理学，而用凝集法评估血清C反应蛋白（CRP）水平。通过使用自动血细胞计数器和化学分析仪评估炎症细胞和其他血液学参数。测定血液中的类风湿因子（Rf）和溶酶体酶水平。结果表明，与患病组相比，（提取物）显著降低了CPR水平、宏观关节炎评分和类风湿因子。组织病理学评估显示，植物提取物显著减轻了骨侵蚀、关节炎症和血管翳形成。（提取物）治疗显著抑制了酸性磷酸酶、β-半乳糖苷酶、β-葡萄糖醛酸酶、N-乙酰氨基葡萄糖苷酶和胶原酶的水平。此外，（提取物）显著提高了患病组中降低的血红蛋白（HB）水平和红细胞（RBCs）计数。植物提取物治疗也显著降低了总白细胞、血小板、中性粒细胞、淋巴细胞和单核细胞的升高计数。比较分析表明，与低剂量相比，高剂量的（提取物）对类风湿性关节炎的改善效果更佳。总之，（腰果叶提取物）具有显著的抗关节炎潜力，这可能归因于对溶酶体酶和胶原酶水平的抑制。
