Department of Chinese Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, 518028, China.
Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
J Ovarian Res. 2021 Dec 8;14(1):172. doi: 10.1186/s13048-021-00923-9.
To investigate the relationship between vaginal microbial community structure and premature ovarian insufficiency (POI).
Twenty-eight women with POI and 12 healthy women were recruited at Shenzhen Maternity and Child Healthcare Hospital between August and September 2020. Blood samples were collected for glucose tests and detection of sex hormone levels and vaginal secretions were collected for microbial group determination. Vaginal microbial community profiles were analysed by 16S rRNA gene sequencing using the Illumina MiSeq system (Illumina Inc., San Diego, CA, USA).
Compared to the controls, the serum levels of follicle-stimulating hormone, luteinizing hormone, testosterone, and the follicle-stimulating hormone/luteinizing hormone ratio, significantly increased, and oestradiol and anti-Müllerian hormone levels significantly decreased in women with POI. Higher weighted UniFrac values were observed in women with POI than in healthy women. Bacteria in the genera Lactobacillus, Brevundimonas, and Odoribacter were more abundant in the microbiomes of healthy women, while the quantity of bacteria in the genus Streptococcus was significantly increased in the microbiomes of women with POI. Moreover, these differences in microbes in women with POI were closely related to follicle-stimulating hormone, luteinizing hormone, oestradiol, and anti-Müllerian hormone levels and to the follicle-stimulating hormone/luteinizing hormone ratio.
Women with POI had altered vaginal microbial profiles compared to healthy controls. The alterations in their microbiomes were associated with serum hormone levels. These results will improve our understanding of the vaginal microbial community structure in women with POI.
CHICTR, ChiCTR2000029576 . Registered 3 August 2020 - Retrospectively registered, https://www.chictr.org.cn/showproj.aspx?proj=48844 .
研究阴道微生物群落结构与卵巢早衰（POI）之间的关系。
2020 年 8 月至 9 月，在深圳市妇幼保健院招募了 28 名 POI 患者和 12 名健康女性。采集血样进行葡萄糖检测和性激素水平检测，采集阴道分泌物进行微生物群测定。采用 Illumina MiSeq 系统（Illumina Inc.，美国圣地亚哥）对 16S rRNA 基因进行测序，分析阴道微生物群落谱。
与对照组相比，POI 患者的血清卵泡刺激素、黄体生成素、睾酮和卵泡刺激素/黄体生成素比值明显升高，雌二醇和抗苗勒管激素水平明显降低。POI 患者的加权 UniFrac 值高于健康女性。健康女性的微生物组中，乳杆菌属、短双歧杆菌属和恶臭杆菌属的细菌更为丰富，而 POI 患者的微生物组中，链球菌属的细菌数量明显增加。此外，POI 患者微生物的这些差异与卵泡刺激素、黄体生成素、雌二醇和抗苗勒管激素水平以及卵泡刺激素/黄体生成素比值密切相关。
与健康对照组相比，POI 患者的阴道微生物谱发生了改变。他们的微生物组的改变与血清激素水平有关。这些结果将提高我们对 POI 患者阴道微生物群落结构的认识。
ChiCTR2000029576。注册日期：2020 年 8 月 3 日-回顾性注册，http://www.chictr.org.cn/showproj.aspx?proj=48844。
