Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 845 Lingshan Road, Shanghai, 200135, China.
Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, 200135, China.
BMC Microbiol. 2020 Jul 29;20(1):230. doi: 10.1186/s12866-020-01918-0.
Primary ovarian failure (POF) is defined as follicular failure in women of reproductive age. Although many factors are speculated to contribute to the occurrence of POF, the exact aetiology remains unclear. Moreover, alterations in the microbiome of patients with POF are poorly studied.
This study investigated the vaginal microbiota of 22 patients with POF and 29 healthy individuals. High-throughput Illumina MiSeq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene was used to evaluate the relationships between the vaginal flora and clinical characteristics of POF. Different from results of previous studies, we found that the diversity and richness of the vaginal flora of patients with POF was significantly different from those of healthy controls. Comparison of the vaginal flora of patients with POF with that of menopausal women revealed that the relative abundance of Lactobacillus was significantly reduced in the latter. A reduced abundance of Lactobacillus was furthermore associated with a lower pregnancy success rate. Of particular interest is that L. gallinarum especially appeared to be beneficially associated with reproductive-related indicators (FSH, E2, AMH, PRL) whilst L. iners appeared to have a detrimental effect. The result of the present study may enable the identification of microbiota associated with POF, however, further investigations of differences in the microbiota in the context of POF will enable a deeper understanding of the disease pathogenesis that involves modification of the vaginal microbiota.
The present study identified the microbiota associated with POF. Further investigations on the differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involves modification of the vaginal microbiota.
原发性卵巢功能不全（POF）定义为育龄期妇女的卵泡衰竭。尽管许多因素被推测与 POF 的发生有关，但确切的病因仍不清楚。此外，POF 患者的微生物组变化研究甚少。
本研究调查了 22 例 POF 患者和 29 例健康个体的阴道微生物群。使用靶向 16S 核糖体 RNA（rRNA）基因 V3-V4 区的高通量 Illumina MiSeq 测序来评估阴道菌群与 POF 临床特征之间的关系。与以往研究结果不同，我们发现 POF 患者阴道菌群的多样性和丰富度与健康对照组有显著差异。与绝经后妇女的阴道菌群相比，POF 患者的阴道菌群中乳杆菌的相对丰度明显降低。乳杆菌丰度降低与妊娠成功率降低有关。特别有趣的是，L. gallinarum 似乎与生殖相关指标（FSH、E2、AMH、PRL）呈正相关，而 L. iners 似乎有不利影响。本研究的结果可能有助于识别与 POF 相关的微生物群，但进一步研究 POF 背景下微生物群的差异将使我们更深入地了解涉及阴道微生物群改变的疾病发病机制。
本研究确定了与 POF 相关的微生物群。进一步研究 POF 背景下微生物群的差异将提高我们对涉及阴道微生物群改变的疾病发病机制的理解。
