Pflüger K H, Probeck H D, Adler G, Stach-Machado D, Kapmeyer H, Havemann K
Blut. 1986 Aug;53(2):89-100. doi: 10.1007/BF00321091.
The cell line 5637 which originated from a human urinary bladder carcinoma is known to produce GM-CSF and Multi-CSF ectopically. Determination of cell surface antigens defined by monoclonal antibodies was recently reported. Here we report on the ultrastructure and karyology of this CSF secreting cell line. At the ultrastructural level the monolayer in vitro culture and the solid tumors formed in nude mice showed all characteristics consistent with a well-differentiated transitional cell carcinoma (TCC). A subclone was found to grow in suspension and did not secrete any CSF activity. High resolution chromosome analysis revealed chromosomal abnormalities which agreed only in few particulars with nonrandom chromosomal aberrations usually found in TCC. Analysis of the cytogenetic results showed that nearly all structural abnormalities present are known to be associated with acute or chronic human leukemia. The possibility that the ectopic production of CSF in this cell line may be correlated to one or more of the described chromosomal aberrations is discussed.
源自人膀胱癌的5637细胞系已知可异位产生粒细胞巨噬细胞集落刺激因子（GM-CSF）和多集落刺激因子（Multi-CSF）。最近报道了用单克隆抗体对细胞表面抗原的测定。在此，我们报告该分泌集落刺激因子（CSF）的细胞系的超微结构和核型。在超微结构水平上，体外单层培养物和裸鼠体内形成的实体瘤显示出与高分化移行细胞癌（TCC）一致的所有特征。发现一个亚克隆以悬浮形式生长且不分泌任何CSF活性。高分辨率染色体分析揭示了染色体异常，这些异常仅在少数细节上与通常在TCC中发现的非随机染色体畸变一致。细胞遗传学结果分析表明，几乎所有存在的结构异常都已知与急性或慢性人类白血病有关。讨论了该细胞系中CSF的异位产生可能与上述一种或多种染色体畸变相关的可能性。
