• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种分泌用于CFU-GEMM培养的刺激因子的细胞系。

A cell line secreting stimulating factors for CFU-GEMM culture.

作者信息

Myers C D, Katz F E, Joshi G, Millar J L

出版信息

Blood. 1984 Jul;64(1):152-5.

PMID:6733267
Abstract

The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned medium has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. We describe the use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cultures and show that the properties of this conditioned medium closely mimic those of PHA-LCM in two separate CFU-GEMM culture systems.

摘要

多能造血干细胞在体外培养时对生长条件要求苛刻。传统上，植物血凝素刺激的白细胞条件培养基一直被用于提供培养人类多能造血祖细胞所需的未明确生长因子。我们描述了使用膀胱癌细胞系5637条件培养基替代CFU - GEMM培养中的PHA - LCM，并表明在两个独立的CFU - GEMM培养系统中，这种条件培养基的特性与PHA - LCM的特性非常相似。

相似文献

1
A cell line secreting stimulating factors for CFU-GEMM culture.一种分泌用于CFU-GEMM培养的刺激因子的细胞系。
Blood. 1984 Jul;64(1):152-5.
2
Studies of human pluripotential hemopoietic stem cells (CFU-GEMM) in vitro.人多能造血干细胞（CFU-GEMM）的体外研究。
Blood. 1981 Aug;58(2):309-16.
3
Interference of a human leukocyte interferon preparation with stimulatory activities in leukocyte-conditioned medium for human hematopoietic stem cells.人白细胞干扰素制剂对人造血干细胞白细胞条件培养基中刺激活性的干扰作用。
Int J Cell Cloning. 1984 Sep;2(5):286-95.
4
Enhancement of haemopoietic stem cell adherence to bone-marrow derived stroma by phyto-haemagglutinin treated leucocyte conditioned medium (PHA-LCM).经植物血凝素处理的白细胞条件培养基（PHA-LCM）增强造血干细胞对骨髓源性基质的黏附。
Indian J Exp Biol. 1994 Apr;32(4):225-8.
5
Response of human myeloid leukemia cells to various sources of colony-stimulating activity and phytohemagglutinin-conditioned medium.人髓性白血病细胞对各种集落刺激活性来源及植物血凝素条件培养基的反应。
Cancer Res. 1983 May;43(5):2350-7.
6
Pluripotent hemopoietic progenitors (CFU-GEMM) in chronic myelogenous leukemia.慢性粒细胞白血病中的多能造血祖细胞（CFU - GEMM）
Int J Cell Cloning. 1983 Sep;1(4):230-9. doi: 10.1002/stem.5530010404.
7
Hemopoietic colony growth-promoting activities in the plasma of bone marrow transplant recipients.骨髓移植受者血浆中的造血集落生长促进活性。
J Clin Invest. 1988 Jul;82(1):255-61. doi: 10.1172/JCI113579.
8
Stimulatory activity of PHA-LCM for normal human hemopoietic progenitors and leukemic blast cell precursors: separation by isoelectric focusing.PHA-LCM对正常人造血祖细胞和白血病原始细胞前体的刺激活性：通过等电聚焦进行分离
J Supramol Struct Cell Biochem. 1981;15(1):41-8. doi: 10.1002/jsscb.1981.380150105.
9
Lymphokine(s) from isolated T lymphocyte subpopulations support multilineage hematopoietic colony and megakaryocytic colony formation.来自分离的T淋巴细胞亚群的淋巴细胞因子支持多谱系造血集落和巨核细胞集落形成。
Blood. 1986 Nov;68(5):991-5.
10
Canine stem cell factor (c-kit ligand) supports the survival of hematopoietic progenitors in long-term canine marrow culture.犬干细胞因子（c-kit配体）在长期犬骨髓培养中支持造血祖细胞的存活。
Exp Hematol. 1992 Oct;20(9):1118-24.

引用本文的文献

1
A validated cellular biobank for β-thalassemia.一个经过验证的β地中海贫血细胞生物样本库。
J Transl Med. 2016 Sep 2;14(1):255. doi: 10.1186/s12967-016-1016-4.
2
Purification and characterization of two recombinant human granulocyte colony-stimulating factor glycoforms. Pharmacokinetic and activity studies of single-dose administration in mice.两种重组人粒细胞集落刺激因子糖型的纯化与表征。小鼠单剂量给药的药代动力学和活性研究。
Mol Biotechnol. 1999 Apr;11(2):117-28. doi: 10.1007/BF02915805.
3
An assessment of myeloid colony-forming-cell generation in liquid human bone marrow cultures: influence of accessory cells and cytokines IL-1 alpha, beta and IL-6.
人骨髓液体培养中髓系集落形成细胞生成的评估：辅助细胞及细胞因子IL-1α、β和IL-6的影响
Int J Exp Pathol. 1993 Apr;74(2):173-80.
4
Verapamil potentiation of melphalan cytotoxicity and cellular uptake in murine fibrosarcoma and bone marrow.维拉帕米增强美法仑对小鼠纤维肉瘤和骨髓的细胞毒性及细胞摄取。
Br J Cancer. 1985 Dec;52(6):813-22. doi: 10.1038/bjc.1985.264.
5
Incidence of the 15q+;17q- chromosome translocation in acute promyelocytic leukaemia (APL).急性早幼粒细胞白血病（APL）中15q+;17q-染色体易位的发生率。
Br J Cancer. 1985 Jul;52(1):55-8. doi: 10.1038/bjc.1985.148.
6
The growth of myelodysplastic bone marrow in long-term cultures.长期培养中骨髓增生异常的生长情况。
Br J Cancer. 1987 Mar;55(3):291-3. doi: 10.1038/bjc.1987.56.
7
Human granulocyte colony stimulating factor.人粒细胞集落刺激因子
Blut. 1987 Mar;54(3):129-36. doi: 10.1007/BF00320366.
8
The role of CFU-GEMM in human hemopoiesis.粒系-红系-单核系-巨核系集落形成单位在人类造血中的作用。
Blut. 1986 Oct;53(4):269-77. doi: 10.1007/BF00320884.
9
Karyotype and ultrastructure of a colony stimulating factor (CSF) producing cell line (5637) originated from a carcinoma of the human urinary bladder.源自人膀胱癌的集落刺激因子（CSF）产生细胞系（5637）的核型和超微结构
Blut. 1986 Aug;53(2):89-100. doi: 10.1007/BF00321091.
10
Pluripoietin alpha: a second human hematopoietic colony-stimulating factor produced by the human bladder carcinoma cell line 5637.多能素α：一种由人膀胱癌细胞系5637产生的第二种人造血集落刺激因子。
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2478-82. doi: 10.1073/pnas.83.8.2478.