Park Sol Lee, Cho Jang Yeon, Kim Su Hyun, Bhatia Shashi Kant, Gurav Ranjit, Park See-Hyoung, Park Kyungmoon, Yang Yung-Hun
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea.
Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul 05029, Korea.
Polymers (Basel). 2021 Dec 4;13(23):4257. doi: 10.3390/polym13234257.
Having the advantage of eco-friendly decomposition, bioplastics could be used to replace petroleum-based plastics. In particular, poly(3-hydroxybutyrate) (PHB) is one of the most commercialized bioplastics, however, necessitating the introduction of PHB-degrading bacteria for its effective disposal. In this study, sp. SOL66 (94.18% 16S rRNA with similarity to ) demonstrated the highest degradation activity among five newly screened genus strains. sp. SOL66 showed a rapid degradation yield, reaching 98% in 4 days, as monitored by laboratory scale, gas chromatography-mass spectrometry, scanning electron microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The PHB film was completely degraded within 7 days at 37 °C in the presence of 3% NaCl. When 1% xylose and 0.4% ammonium sulfate were added, the degradation activity increased by 17% and 24%, respectively. In addition, this strain showed biodegradability on pellets of poly(3-hydroxybutyrate--4-hydroxybutyrate), as confirmed by weight loss and physical property changes. We confirmed that sp. SOL66 has a great ability to degrade PHB, and has rarely been reported to date.
生物塑料具有生态友好型分解的优势，可用于替代石油基塑料。特别是聚（3-羟基丁酸酯）（PHB）是最商业化的生物塑料之一，然而，需要引入PHB降解细菌才能对其进行有效处理。在本研究中，菌株SOL66（16S rRNA与[某菌株]的相似度为94.18%）在新筛选的5株[某菌属]菌株中表现出最高的降解活性。通过实验室规模的气相色谱-质谱联用仪、扫描电子显微镜、凝胶渗透色谱和傅里叶变换红外光谱监测发现，菌株SOL66降解效率高，4天内降解率达98%。在37℃、3%NaCl存在的条件下，PHB薄膜在7天内完全降解。当添加1%木糖和0.4%硫酸铵时,降解活性分别提高了17%和24%。此外，通过重量损失和物理性质变化证实，该菌株对聚（3-羟基丁酸酯-co-4-羟基丁酸酯）颗粒也具有生物降解性。我们证实，菌株SOL66具有很强的PHB降解能力，且迄今为止鲜有报道。
Zotero 插件
