Cho Jang Yeon, Kim Su Hyun, Jung Hee Ju, Cho Do Hyun, Kim Byung Chan, Bhatia Shashi Kant, Ahn Jungoh, Jeon Jong-Min, Yoon Jeong-Jun, Lee Jongbok, Yang Yung-Hun
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea.
Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Cheongju 28116, Korea.
Polymers (Basel). 2022 Sep 1;14(17):3625. doi: 10.3390/polym14173625.
As a biodegradable plastic, polyhydroxybutyrate (PHB) has relatively poor mechanical properties, preventing its wider use. Various plasticizers have been studied to improve the mechanical properties of PHB; however, due to the slow degradation speed in the soil environment and lack of evaluation methods, studies on the degradation of PHB with plasticizers are rarely reported. In this study, by applying sp. SOL66, which is able to degrade PHB very quickly, a benign plasticizer was evaluated with good properties and good degradability, not inhibiting microbial activities. Eight different plasticizers were applied with PHB and sp. SOL66, PHB film containing 10% and 20% tributyl citrate showed significant biodegradability of PHB. It was confirmed that tributyl citrate could increase the speed of PHB degradation by sp. SOL66 by 88% at 1 day, although the degree of degradation was similar after 3 days with and without tributyl citrate. By the analysis of microbial degradation, physical, chemical, and mechanical properties, tributyl citrate was shown not only to improve physical, chemical, and mechanical properties but also the speed of microbial degradation.
作为一种可生物降解的塑料，聚羟基丁酸酯（PHB）的机械性能相对较差，这限制了其更广泛的应用。人们研究了各种增塑剂来改善PHB的机械性能；然而，由于在土壤环境中降解速度缓慢且缺乏评估方法，关于含增塑剂的PHB降解的研究鲜有报道。在本研究中，通过应用能够快速降解PHB的菌株sp. SOL66，评估了一种性能良好且具有良好降解性、不抑制微生物活性的良性增塑剂。将八种不同的增塑剂与PHB和菌株sp. SOL66一起使用，含有10%和20%柠檬酸三丁酯的PHB薄膜显示出显著的PHB生物降解性。证实柠檬酸三丁酯在第1天可使菌株sp. SOL66对PHB的降解速度提高88%，尽管在有和没有柠檬酸三丁酯的情况下，3天后的降解程度相似。通过对微生物降解、物理、化学和机械性能的分析，表明柠檬酸三丁酯不仅能改善物理、化学和机械性能，还能提高微生物降解速度。
