Orlandella Francesca Maria, Auletta Luigi, Greco Adelaide, Zannetti Antonella, Salvatore Giuliana
IRCCS SDN, 80143 Naples, Italy.
Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, 80145 Naples, Italy.
Cancers (Basel). 2021 Nov 30;13(23):6020. doi: 10.3390/cancers13236020.
We have conducted a systematic review focusing on the advancements in preclinical molecular imaging to study the delivery and therapeutic efficacy of miRNAs in mouse models of breast cancer.
A systematic review of English articles published in peer-reviewed journals using PubMed, EMBASE, BIOSIS™ and Scopus was performed. Search terms included breast cancer, mouse, mice, microRNA(s) and miRNA(s).
From a total of 2073 records, our final data extraction was from 114 manuscripts. The most frequently used murine genetic background was Balb/C (46.7%). The most frequently used model was the IV metastatic model (46.8%), which was obtained via intravenous injection (68.9%) in the tail vein. Bioluminescence was the most used frequently used tool (64%), and was used as a surrogate for tumor growth for efficacy treatment or for the evaluation of tumorigenicity in miRNA-transfected cells (29.9%); for tracking, evaluation of engraftment and for response to therapy in metastatic models (50.6%).
This review provides a systematic and focused analysis of all the information available and related to the imaging protocols with which to test miRNA therapy in an in vivo mice model of breast cancer, and has the purpose of providing an important tool to suggest the best preclinical imaging protocol based on available evidence.
我们进行了一项系统综述，重点关注临床前分子成像的进展，以研究微小RNA（miRNA）在乳腺癌小鼠模型中的递送和治疗效果。
使用PubMed、EMBASE、BIOSIS™和Scopus对同行评审期刊上发表的英文文章进行系统综述。检索词包括乳腺癌、小鼠、微RNA（s）和微小RNA（s）。
从总共2073条记录中，我们最终的数据提取来自114篇手稿。最常用的小鼠遗传背景是Balb/C（46.7%）。最常用的模型是IV转移模型（46.8%），它是通过尾静脉静脉注射（68.9%）获得的。生物发光是最常用的工具（64%），在miRNA转染细胞中用作疗效治疗的肿瘤生长替代指标或用于评估致瘤性（29.9%）；在转移模型中用于追踪、评估植入和对治疗的反应（50.6%）。
本综述对所有可用的以及与在乳腺癌体内小鼠模型中测试miRNA治疗的成像方案相关的信息进行了系统且有针对性的分析，目的是提供一个重要工具，根据现有证据推荐最佳的临床前成像方案。
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