针对一种主要糖蛋白免疫原的单克隆抗体介导阴道毛滴虫的补体非依赖性差异溶解。
Monoclonal antibody to a major glycoprotein immunogen mediates differential complement-independent lysis of Trichomonas vaginalis.
作者信息
Alderete J F, Kasmala L
出版信息
Infect Immun. 1986 Sep;53(3):697-9. doi: 10.1128/iai.53.3.697-699.1986.
Abstract
An immunoglobulin G type 2a (IgG2a) monoclonal antibody (MAb), designated C20A3, which reacts with a highly immunogenic trichomonad membrane glycoprotein (approximately 270,000 daltons), produced complement-independent cytolysis of Trichomonas vaginalis organisms. Time- and temperature-dependent lysis of parasites was observed following incubation of washed, live T. vaginalis with certain concentrations of C20A3 IgG. Differential killing of trichomonal isolates and clones of a given isolate by C20A3 was dependent on the presence of the glycoprotein antigen on the parasite surface.
摘要
一种免疫球蛋白G2a（IgG2a）单克隆抗体（MAb），命名为C20A3，它与一种高度免疫原性的滴虫膜糖蛋白（约270,000道尔顿）发生反应，可对阴道毛滴虫产生不依赖补体的细胞溶解作用。在用一定浓度的C20A3 IgG孵育洗涤后的活阴道毛滴虫后，观察到寄生虫出现了时间和温度依赖性的裂解。C20A3对滴虫分离株和给定分离株的克隆的差异性杀伤取决于寄生虫表面糖蛋白抗原的存在。
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