State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, China.
Mol Plant Microbe Interact. 2021 Dec;34(12):1378-1389. doi: 10.1094/MPMI-05-21-0117-R. Epub 2021 Dec 10.
Fusaricidins produced by are important lipopeptide antibiotics against fungi. The (fusaricidin biosynthesis) operon is responsible for synthesis of fusaricidins. However, the regulation mechanisms of fusaricidin biosynthesis remain to be fully clarified. In this study, we revealed that fusaricidin production is controlled by a complex regulatory network including KinB-Spo0A-AbrB. Evidence suggested that the regulator AbrB represses the transcription of the gene cluster by direct binding to the promoter, in which the sequences (5'-AATTTTAAAATAAATTTTGTGATTT-3') located from -136 to -112 bp relative to the transcription start site is required for this repression. Spo0A binds to the promoter that contains the Spo0A-binding sequences (5'-TGTCGAA-3', 0A box) and in turn prevents the further transcription of . The decreasing concentration of AbrB allows for the derepression of the promoter repressed by AbrB. The genome of . WLY78 contains two orthologs (named Kin1508 and Kin4833) of KinB, but only Kin4833 activates sporulation and fusaricidin production, indicating that this kinase may be involved in phosphorylating Spo0A to initiate sporulation and regulate the transcription. Our results reveal that Kin4833 (KinB), Spo0A, and AbrB are involved in regulation of fusaricidin production and a signaling mechanism that links fusaricidin production and sporulation.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
由 产生的 fusaricidins 是针对真菌的重要脂肽抗生素。 （fusaricidin 生物合成）操纵子负责 fusaricidins 的合成。然而，fusaricidin 生物合成的调控机制仍有待充分阐明。在这项研究中，我们揭示了 fusaricidin 的产生受包括 KinB-Spo0A-AbrB 在内的复杂调控网络控制。有证据表明，调节剂 AbrB 通过直接结合 启动子来抑制 基因簇的转录，该启动子中位于转录起始位点前-136 至-112bp 的序列（5'-AATTTTAAAATAAATTTTGTGATTT-3'）对于这种抑制是必需的。Spo0A 结合到包含 Spo0A 结合序列（5'-TGTCGAA-3'，0A 盒）的 启动子上，从而阻止 的进一步转录。AbrB 浓度的降低允许被 AbrB 抑制的 启动子去抑制。 . WLY78 的基因组包含两个 KinB 的同源物（命名为 Kin1508 和 Kin4833），但只有 Kin4833 激活孢子形成和 fusaricidin 产生，表明该激酶可能参与磷酸化 Spo0A 以启动孢子形成并调节 转录。我们的结果表明，Kin4833（KinB）、Spo0A 和 AbrB 参与 fusaricidin 产生的调控以及将 fusaricidin 产生与孢子形成联系起来的信号机制。[公式：见正文]版权所有 © 2021 作者。这是一份在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
