固氮菌与：来自植物根际的两个新物种。

Nitrogen-Fixing and : Two Novel Species from Plant Rhizospheres.

作者信息

Zhang Weilong, Gao Miao, Hu Rui, Shang Yimin, Liu Minzhi, Lan Peichun, Jiao Shuo, Wei Gehong, Chen Sanfeng

机构信息

College of Biological Sciences, China Agricultural University, Beijing 100193, China.

State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Microorganisms. 2024 Dec 12;12(12):2561. doi: 10.3390/microorganisms12122561.

DOI:10.3390/microorganisms12122561

PMID:39770764

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11676665/

Abstract

Two strains, M1 and H32 with nitrogen-fixing ability, were isolated from the rhizospheres of different plants. Genome sequence analysis showed that a (trogen ixation) gene cluster composed of nine genes () was conserved in the two strains. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strains M1 and H32 are members of the genus . Strains M1 and H32 had 97% similarity in the 16S rRNA gene sequences. Strain M1 had the highest similarity (97.25%) with LAM 0504T in the 16S rRNA gene sequences. Strain H32 had the highest similarity (97.48%) with TCIP 101062T in the 16S rRNA gene sequences. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain M1 and its closest member were 78.17% and 22.3%, respectively. ANI and dDDH values between strain H32 and its closest member were 88.94% and 66.02%, respectively. The predominant fatty acid of both strains is anteiso-C15:0. The major polar lipids of both strains are DPG (diphosphatidylglycerol) and PG (phosphatidylglycerol). The predominant isoprenoid quinone of both strains is MK-7. With all the phylogenetic and phenotypic divergency, two novel species sp. nov and sp. nov are proposed with the type strain M1 [=GDMCC (Guangdong Culture Collection Centre of Microbiology) 1.4871 = JCM (Japan Collection of Microorganisms) 37487] and with type strain H32 (=GDMCC 1.4872 = JCM37488).

摘要

从不同植物的根际分离出两株具有固氮能力的菌株，M1和H32。基因组序列分析表明，由九个基因组成的一个（固氮）基因簇在这两株菌株中是保守的。基于16S rRNA基因序列的系统发育分析表明，菌株M1和H32是（某属）属的成员。菌株M1和H32的16S rRNA基因序列相似度为97%。菌株M1在16S rRNA基因序列中与（某菌株名）LAM 0504T的相似度最高（97.25%）。菌株H32在16S rRNA基因序列中与（某菌株名）TCIP 101062T的相似度最高（97.48%）。菌株M1与其最接近的成员（某菌株名）之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为78.17%和22.3%。菌株H32与其最接近的成员（某菌株名）之间的ANI和dDDH值分别为88.94%和66.02%。两株菌株的主要脂肪酸均为anteiso-C15:0。两株菌株的主要极性脂均为DPG（双磷脂酰甘油）和PG（磷脂酰甘油）。两株菌株的主要类异戊二烯醌均为MK-7。综合所有系统发育和表型差异，提出两个新物种（新物种名1）sp. nov和（新物种名2）sp. nov，模式菌株分别为M1 [=GDMCC（广东微生物培养物保藏中心）1.4871 = JCM（日本微生物菌种保藏中心）37487]和模式菌株H32（=GDMCC 1.4872 = JCM37488）。