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印度花椰菜(甘蓝型油菜 var. botrytis L.)不同成熟组的农艺形态和分子多样性。

Agro-morphological and molecular diversity in different maturity groups of Indian cauliflower (Brassica oleracea var. botrytis L.).

机构信息

Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, India.

ICAR Research Complex for North Eastern Hill Region, Barapani, Meghalaya, India.

出版信息

PLoS One. 2021 Dec 10;16(12):e0260246. doi: 10.1371/journal.pone.0260246. eCollection 2021.

Abstract

The present study analysed the molecular and agro-morphological diversity in a set of 92 diverse cauliflower genotypes and two each of cabbage and broccoli. Field evaluation of the genotypes was done in randomized block design (RBD) at two locations (i.e. IARI, New Delhi and ICAR-RC-NEH Region, Barapani) during Rabi2019-20. Genotypes showed variation for all the eight observed traits at both locations and, the differences in early and snowball groups were distinct. Pusa Meghna, DC-33-8, Pusa Kartiki and CC-14 were earliest for curd initiation. Genotypes showed higher values for curd traits at Delhi. Molecular diversity was detected with 90 polymorphic simple sequence repeats (SSR). Number of alleles ranged from 1 to 9 with mean value of 2.16 and the highest polymorphic information content (PIC) value was observed for primer BoGMS0742 (0.68) with a mean value of 0.18. Cluster analysis using agro-morphological traits substantiated classification of the genotypes for maturity groups. However, SSR analysis revealed four clusters and with a composite pattern of genotype distribution. STRUCTURE analysis also supported the admixture and four subpopulations. The studyindicates for introgression of genetic fragments across the maturity groups, thereby, potential for use in further genetic improvement and heterosis breeding.

摘要

本研究分析了 92 个不同的花椰菜基因型和 2 个白菜和西兰花基因型的分子和农艺形态多样性。在 2019-20 年冬季,这些基因型在两个地点(即 IARI,新德里和 ICAR-RC-NEH 地区,Barapani)的随机区组设计(RBD)中进行了田间评估。基因型在两个地点的所有八个观察性状上均表现出变异,早期和雪球组之间的差异明显。Pusa Meghna、DC-33-8、Pusa Kartiki 和 CC-14 是最早出现花蕾的品种。基因型在德里表现出更高的花蕾性状值。利用 90 个多态性简单重复序列(SSR)检测到分子多样性。等位基因数范围为 1 至 9,平均值为 2.16,引物 BoGMS0742(0.68)的最高多态信息含量(PIC)值为 0.18。使用农艺形态性状进行聚类分析证实了基因型的成熟组分类。然而,SSR 分析显示有四个聚类,基因型的分布具有综合模式。STRUCTURE 分析也支持混合和四个亚群。该研究表明遗传片段在不同成熟组之间的渗入,从而有可能进一步用于遗传改良和杂种优势育种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d4a/8664203/5e3248415577/pone.0260246.g001.jpg

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