Malek T R, Ortega G, Chan C, Kroczek R A, Shevach E M
J Exp Med. 1986 Sep 1;164(3):709-22. doi: 10.1084/jem.164.3.709.
The Ly-6 locus controls the expression and/or encodes for alloantigenic specificities found primarily on subpopulations of murine T and B lymphocytes. We have recently identified and characterized a new rat mAb, D7, that recognizes a nonpolymorphic Ly-6 specificity. After crosslinking by anti-Ig reagents or by Fc receptor-bearing accessory cells, mAb D7 could induce IL-2 production from T cell hybridomas, and in the presence of PMA could trigger a vigorous proliferative response in resting peripheral T cells. The addition of mAb D7 to cultures of antigen- and alloantigen-, but not mitogen-stimulated T cells resulted in a marked augmentation of the proliferative response. A number of other well-characterized mAbs to Ly-6 locus products could also stimulate a T cell proliferative response after crosslinking by anti-Ig and in the presence of PMA. These results strongly suggest that Ly-6 molecules may play a critical role in the T cell activation cascade, either as receptors for an unidentified soluble or cell-associated ligand or as transducing molecules that modulate signals initiated by antigen stimulation of the T3-Ti complex.
Ly-6基因座控制主要在小鼠T和B淋巴细胞亚群上发现的同种异体抗原特异性的表达和/或编码。我们最近鉴定并表征了一种新的大鼠单克隆抗体D7，它识别一种非多态性的Ly-6特异性。在用抗Ig试剂或带有Fc受体的辅助细胞交联后，单克隆抗体D7可诱导T细胞杂交瘤产生IL-2，并且在有佛波酯（PMA）存在的情况下可触发静息外周T细胞的强烈增殖反应。将单克隆抗体D7添加到抗原和同种异体抗原刺激而非丝裂原刺激的T细胞培养物中，导致增殖反应显著增强。许多其他已充分表征的针对Ly-6基因座产物的单克隆抗体在通过抗Ig交联并在有PMA存在的情况下也可刺激T细胞增殖反应。这些结果强烈表明，Ly-6分子可能在T细胞激活级联反应中起关键作用，要么作为未鉴定的可溶性或细胞相关配体的受体，要么作为调节由T3-Ti复合物的抗原刺激引发的信号的转导分子。
