Speciale Immacolata, Notaro Anna, Garcia-Vello Pilar, Di Lorenzo Flaviana, Armiento Samantha, Molinaro Antonio, Marchetti Roberta, Silipo Alba, De Castro Cristina
Department of Agricultural Sciences, University of Naples, 80055 Portici, Italy.
Department of Chemical Sciences, University of Naples, 80126 Naples, Italy.
Carbohydr Polym. 2022 Feb 1;277:118885. doi: 10.1016/j.carbpol.2021.118885. Epub 2021 Nov 13.
Structural determination of carbohydrates is mostly performed by liquid-state NMR, and it is a demanding task because the NMR signals of these biomolecules explore a rather narrow range of chemical shifts, with the result that the resonances of each monosaccharide unit heavily overlap with those of others, thus muddling their punctual identification. However, the full attribution of the NMR chemical shifts brings great advantages: it discloses the nature of the constituents, the way they are interconnected, in some cases their absolute configuration, and it paves the way to other and more sophisticated analyses. The purpose of this review is to provide a practical guide into this challenging subject. It will drive through the strategy used to assign the NMR data, pinpointing the core information disclosed from each NMR experiment, and suggesting useful tricks for their interpretation, along with other resources pivotal during the study of these biomolecules.
碳水化合物的结构测定大多通过液态核磁共振（NMR）来进行，这是一项艰巨的任务，因为这些生物分子的NMR信号所探索的化学位移范围相当窄，结果是每个单糖单元的共振与其他单元的共振严重重叠，从而使它们的精确识别变得模糊不清。然而，NMR化学位移的完全归属带来了很大的优势：它揭示了成分的性质、它们相互连接的方式，在某些情况下还能揭示它们的绝对构型，并且为其他更复杂的分析铺平了道路。本综述的目的是为这个具有挑战性的主题提供一份实用指南。它将贯穿用于确定NMR数据的策略，指出每个NMR实验所揭示的核心信息，并为其解释提出有用的技巧，以及在研究这些生物分子过程中至关重要的其他资源。
