Fachbereich Physik, Center for Hybrid Nanostructures (CHyN), Universitat Hamburg, 22607, Hamburg, Germany.
Department of Chemical and Pharmaceutical Sciences, INSTM UdR Trieste, University of Trieste, Via Licio Giorgieri 1, 34127, Trieste, Italy.
Nat Commun. 2021 Dec 10;12(1):7208. doi: 10.1038/s41467-021-27406-1.
Carbon nanodots with opposite chirality possess the same major physicochemical properties such as optical features, hydrodynamic diameter, and colloidal stability. Here, a detailed analysis about the comparison of the concentration of both carbon nanodots is carried out, putting a threshold to when differences in biological behavior may be related to chirality and may exclude effects based merely on differences in exposure concentrations due to uncertainties in concentration determination. The present study approaches this comparative analysis evaluating two basic biological phenomena, the protein adsorption and cell internalization. We find how a meticulous concentration error estimation enables the evaluation of the differences in biological effects related to chirality.
具有相反手性的碳纳米点具有相同的主要物理化学性质，如光学特性、水动力直径和胶体稳定性。在这里，对两种碳纳米点浓度的比较进行了详细分析，确定了生物行为差异可能与手性有关的阈值，并且可以排除仅仅由于浓度测定的不确定性而导致的暴露浓度差异引起的影响。本研究通过评估两种基本的生物学现象，即蛋白质吸附和细胞内化，来进行这种比较分析。我们发现，如何进行细致的浓度误差估计可以评估与手性相关的生物学效应的差异。
