流式细胞术分析骨髓增生异常：分析前和技术问题-欧洲白血病网络的建议。
Flow cytometric analysis of myelodysplasia: Pre-analytical and technical issues-Recommendations from the European LeukemiaNet.
机构信息
Laboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Department of Laboratory Medicine - Laboratory for Hematology, Radboudumc, Nijmegen, The Netherlands.
出版信息
Cytometry B Clin Cytom. 2023 Jan;104(1):15-26. doi: 10.1002/cyto.b.22046. Epub 2021 Dec 11.
BACKGROUND
Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures.
METHODS
Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group.
RESULTS
We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies.
CONCLUSIONS
These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.
背景
流式细胞术（FCM）有助于诊断和预后分层疑似或确诊的骨髓增生异常综合征（MDS）患者。在过去几年中，FCM 领域在技术问题（包括软件和硬件）和分析前程序方面取得了重大进展。
方法
根据 13 次欧洲白血病网国际 MDS FCM 工作组年会的数据和专家讨论，提出了建议。
结果
我们在此报告有关（1）最佳样本处理和处理方法，（2）抗体面板和荧光染料，以及（3）当前硬件技术的经验和建议。
结论
这些建议将支持和促进 FCM 检测在 MDS 患者诊断中的适当应用。需要进一步的标准化和协调，以便将 FCM 纳入 MDS 诊断评估的日常实践中。
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